Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24153||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonemodified pEV3S (pLG-033)
Backbone manufacturerMatthias et al, Nucleic Acid Research (1989).
- Backbone size w/o insert (bp) 5120
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1600
Entrez GeneRELA (a.k.a. CMCU, NFKB3, p65)
/ Fusion Protein
- T7 gp10 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
FOr more details for this expression construct as well as other mutants, please contact Dr Lin-Feng CHen ([email protected])
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:T7-RelA(S276A) was a gift from Warner Greene (Addgene plasmid # 24153 ; http://n2t.net/addgene:24153 ; RRID:Addgene_24153)
For your References section:NF-kappaB RelA phosphorylation regulates RelA acetylation. Chen LF, Williams SA, Mu Y, Nakano H, Duerr JM, Buckbinder L, Greene WC. Mol Cell Biol. 2005 Sep . 25(18):7966-75. 10.1128/MCB.25.18.7966-7975.2005 PubMed 16135789
Map uploaded by the depositor.