Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pLenti6-MK1-EHMT2-V5
(Plasmid #31113)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31113 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLenti6/V5-D-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6963
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    euchromatic histone-lysine N-methyltransferase 2
  • Alt name
    EHMT2
  • Alt name
    G9a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3633
  • Mutation
    Stop codon mutated to Ala
  • GenBank ID
    NM_006709
  • Entrez Gene
    EHMT2 (a.k.a. BAT8, C6orf30, G9A, GAT8, KMT1C, NG36)
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site TOPO (unknown if destroyed)
  • 3′ cloning site TOPO (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer V5
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystem (Mammalian Genome Collection) Accession # BC018718.1
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Spektor TM, Rice JC. Identification and characterization of posttranslational modification-specific binding proteins in vivo by mammalian tethered catalysis. Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):14808-13. Epub 2009 Aug 18.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6-MK1-EHMT2-V5 was a gift from Bernard Futscher (Addgene plasmid # 31113 ; http://n2t.net/addgene:31113 ; RRID:Addgene_31113)