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pLKO-RB1-shRNA63
(Plasmid #25641)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 25641 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1 puro
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RB1-shRNA 63
  • Alt name
    Retinoblastoma tumor susceptibility protein
  • Alt name
    RB1
  • Alt name
    Rb
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    42
  • GenBank ID
    NM_000321
  • Entrez Gene
    RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-RB1-shRNA63 was a gift from Todd Waldman (Addgene plasmid # 25641 ; http://n2t.net/addgene:25641 ; RRID:Addgene_25641)
  • For your References section:

    Pharmacologic inhibition of cyclin-dependent kinases 4 and 6 arrests the growth of glioblastoma multiforme intracranial xenografts. Michaud K, Solomon DA, Oermann E, Kim JS, Zhong WZ, Prados MD, Ozawa T, James CD, Waldman T. Cancer Res. 2010 Apr 15. 70(8):3228-38. 10.1158/0008-5472.CAN-09-4559 PubMed 20354191