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pCRISPRyl Citations (13)

Originally described in: Synthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica.
Schwartz CM, Hussain MS, Blenner M, Wheeldon I ACS Synth Biol. 2015 Dec 29.
PubMed Journal

Articles Citing pCRISPRyl

Developing a piggyBac Transposon System and Compatible Selection Markers for Insertional Mutagenesis and Genome Engineering in Yarrowia lipolytica. Wagner JM, Williams EV, Alper HS. Biotechnol J. 2018 May;13(5):e1800022. doi: 10.1002/biot.201800022. Epub 2018 Mar 25. PubMed
CRISPR-Cas9-Mediated Genome Editing and Transcriptional Control in Yarrowia lipolytica. Schwartz C, Wheeldon I. Methods Mol Biol. 2018;1772:327-345. doi: 10.1007/978-1-4939-7795-6_18. PubMed
Validating genome-wide CRISPR-Cas9 function improves screening in the oleaginous yeast Yarrowia lipolytica. Schwartz C, Cheng JF, Evans R, Schwartz CA, Wagner JM, Anglin S, Beitz A, Pan W, Lonardi S, Blenner M, Alper HS, Yoshikuni Y, Wheeldon I. Metab Eng. 2019 Jun 16;55:102-110. doi: 10.1016/j.ymben.2019.06.007. PubMed
In silico identification of metabolic engineering strategies for improved lipid production in Yarrowia lipolytica by genome-scale metabolic modeling. Kim M, Park BG, Kim EJ, Kim J, Kim BG. Biotechnol Biofuels. 2019 Jul 24;12:187. doi: 10.1186/s13068-019-1518-4. eCollection 2019. PubMed
Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Bruder S, Moldenhauer EJ, Lemke RD, Ledesma-Amaro R, Kabisch J. Biotechnol Biofuels. 2019 Aug 24;12:202. doi: 10.1186/s13068-019-1542-4. eCollection 2019. PubMed
Multiplex Gene Disruption by Targeted Base Editing of Yarrowia lipolytica Genome Using Cytidine Deaminase Combined with the CRISPR/Cas9 System. Bae SJ, Park BG, Kim BG, Hahn JS. Biotechnol J. 2020 Jan;15(1):e1900238. doi: 10.1002/biot.201900238. Epub 2019 Nov 11. PubMed
Application of Random Mutagenesis and Synthetic FadR Promoter for de novo Production of omega-Hydroxy Fatty Acid in Yarrowia lipolytica. Park BG, Kim J, Kim EJ, Kim Y, Kim J, Kim JY, Kim BG. Front Bioeng Biotechnol. 2021 Feb 22;9:624838. doi: 10.3389/fbioe.2021.624838. eCollection 2021. PubMed
Design of Hybrid RNA Polymerase III Promoters for Efficient CRISPR-Cas9 Function. Misa J, Schwartz C, Wheeldon I. Bio Protoc. 2018 Mar 20;8(6):e2779. doi: 10.21769/BioProtoc.2779. eCollection 2018 Mar 20. PubMed
Revisiting the unique structure of autonomously replicating sequences in Yarrowia lipolytica and its role in pathway engineering. Lopez C, Cao M, Yao Z, Shao Z. Appl Microbiol Biotechnol. 2021 Aug 6. pii: 10.1007/s00253-021-11399-4. doi: 10.1007/s00253-021-11399-4. PubMed

Associated Plasmids

Genome-wide functional screens enable the prediction of high activity CRISPR-Cas9 and -Cas12a guides in Yarrowia lipolytica. Baisya D, Ramesh A, Schwartz C, Lonardi S, Wheeldon I. Nat Commun. 2022 Feb 17;13(1):922. doi: 10.1038/s41467-022-28540-0. PubMed
Genome-wide CRISPR-Cas9 screen reveals a persistent null-hyphal phenotype that maintains high carotenoid production in Yarrowia lipolytica. Lupish B, Hall J, Schwartz C, Ramesh A, Morrison C, Wheeldon I. Biotechnol Bioeng. 2022 Dec;119(12):3623-3631. doi: 10.1002/bit.28219. Epub 2022 Sep 22. PubMed
acCRISPR: an activity-correction method for improving the accuracy of CRISPR screens. Ramesh A, Trivedi V, Lee S, Tafrishi A, Schwartz C, Mohseni A, Li M, Lonardi S, Wheeldon I. Commun Biol. 2023 Jun 8;6(1):617. doi: 10.1038/s42003-023-04996-8. PubMed
Characterization of an efficient CRISPR-iCas9 system in Yarrowia lipolytica for the biosynthesis of carotenoids. Chen QH, Qian YD, Niu YJ, Hu CY, Meng YH. Appl Microbiol Biotechnol. 2023 Oct;107(20):6299-6313. doi: 10.1007/s00253-023-12731-w. Epub 2023 Aug 29. PubMed

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