159,258 results

1. plenti guide Thy1.1

   Plasmid

   #128063

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB-rDA3h

   Plasmid

   #208703

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GRAB_ACh3.0

   Plasmid

   #121922

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Has Service

   AAV9

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Casp8

   Plasmid

   #11817

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

   Insert

   Caspase 8 (CASP8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORTMAGE-3

   Plasmid

   #72678

   Purpose

   Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   E32K mutation conferring dominant mutator phenoty…

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDIAI

   Plasmid

   #51655

   Purpose

   E coli genetic modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   apramycin antibiotic resistance gene
   

   Use

   Genetic modification

   Tags

   Expression

   Mutation

   Promoter

   unknown

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIH1-puro-STAT3 shRNA

   Plasmid

   #26596

   Depositor

   Frank Sinicrope

   Article

   Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

   Insert

   STAT3 shRNA (STAT3 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBTet_NADP-Snifit-cyto

   Plasmid

   #113917

   Purpose

   Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBa.Halo-LDLR

    Plasmid

    #162717

    Purpose

    Mammalian expression of human LDLR

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    LDLR (LDLR Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2-nCas9n

    Plasmid

    #47929

    Purpose

    expression of an optimized Cas9 for genome-editing in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    nls-zcas9-nls
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. PM-FRB-mRFP-T2A-FKBP-5-ptase

    Plasmid

    #40896

    Depositor

    Peter Varnai

    Article

    Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

    Insert

    PM-FRB-mRFP-T2A-FKBP-5-ptase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. piggyBAC-5xTAPE-1-BlastR

    Plasmid

    #175808

    Purpose

    Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Insert

    5xTAPE-1 construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-CAG-rtTA3G-IH

    Plasmid

    #214118

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    trTA3G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACBSR-hyg

    Plasmid

    #87830

    Purpose

    Expresses lambda-red system with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    bla

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. P931_CLYBL-CBX3-Cbh-Zeo-TetO-NAIP2

    Plasmid

    #202761

    Purpose

    Encodes four RGC specific transcription factors under the control of a TetOn promoter at the CLYBL safe harbor

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    NEUROG2-p2A-ATOH7-p2A-ISL1-p2A-POU4F2 (NEUROG2, ATOH7, ISL1, POU4F2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

    Plasmid

    #64324

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. nAChR beta2 WT

    Plasmid

    #24272

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR beta2 WT (Chrnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGM1190

    Plasmid

    #69994

    Purpose

    OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_IDH1_p.R132C

    Plasmid

    #81726

    Purpose

    Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R132C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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