165,947 results

1. pBiT3.1-C_AAMP:1-434

   Plasmid

   #211265

   Purpose

   Mammalian expression of full-length human AAMP. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   AAMP:M1-R434 (AAMP Human)
   

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

   Plasmid

   #86424

   Purpose

   PCR tagging module with yeast codon optimized mTurquoise2

   Depositor

   Sue Jaspersen

   Article

   Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

   Insert

   mTurquoise2
   

   Expression

   Yeast

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HsB2AR-mCherry

   Plasmid

   #137785

   Purpose

   Visualization of the Beta-2-adrenergic receptor

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Human beta2-adrenergic receptor (ADRB2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GSK3betaS9A  (1016)

   Plasmid

   #49492

   Purpose

   expresses human Gsk3beta with S9A (constitutively active) mutation

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   GSK3beta S9A (GSK3B Human)
   

   Expression

   Mammalian

   Mutation

   S9A (constitutively active)

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-2TK 14-3-3 sigma GST

   Plasmid

   #11944

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

   Insert

   14-3-3 sigma (SFN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pENTR223-BRLF1(Rta)

   Plasmid

   #195824

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BRLF1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BRLF1 (BRLF1 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. anti-FLAG M2 light chain

   Plasmid

   #175358

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 light chain

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 light chain
   

   Tags

   N-terminal secretion signal peptide (MEAPAQLLFLLL…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FKBP-mCherry

   Plasmid

   #108122

   Purpose

   Recruitable fluorophore

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-eCas9-sgNT

   Plasmid

   #140238

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Expression

    Bacterial

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe_Hyg_cOVA_T2A_mStrawberry

    Plasmid

    #161737

    Purpose

    Expression of cOVA and mStrawberry

    Depositor

    Alec Kimmelman

    Article

    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

    Insert

    cytoplasmic ovalbumin
    

    Use

    Retroviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCK083 desmaMCS:minpromEGFP,exorh:mCherry

    Plasmid

    #200016

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57-GFP-2A-MCS

    Plasmid

    #71783

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBOBI C-FLAG PHGDH

    Plasmid

    #212995

    Purpose

    expresses human PHGDH

    Depositor

    Sheng-cai Lin

    Article

    Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2932

    Plasmid

    #144408

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFAP2A (TFAP2A Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hs.NRAS Q61R

    Plasmid

    #83179

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ITPR1_Halo_N_allele

    Plasmid

    #178161

    Purpose

    Donor vector for endogenous tagging of human ITPR1 at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (ITPR1 Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. p426_Cas9_gRNA-ARS208a

    Plasmid

    #87383

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS208a sequence GTCCGCTAAACAAAAGATCT in yeast chromosome 2.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS208a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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