161,851 results

1. pGEX Cis1-SH2

   Plasmid

   #46417

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Cis1 (SOCS1 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 90-214

   Promoter

   Tac

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

   Plasmid

   #196192

   Purpose

   human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
   

   Use

   Znf donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MC177VSG221RNAi

   Plasmid

   #59730

   Purpose

   For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

   Depositor

   Gloria Rudenko

   Article

   Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

   Insert

   VSG221 RNAi fragment
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   opposing T7 promoters

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPOTv7-blast-nanoluc

   Plasmid

   #179797

   Purpose

   Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

   Depositor

   Keith Gull, Samuel Dean

   Article

   Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

   Insert

   nanoluc
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   read through

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-CLASP2 512-650

   Plasmid

   #24409

   Depositor

   Torsten Wittmann

   Article

   Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

   Insert

   CLASP2 (512-650) (CLASP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CLASP2 deletion mutant with only MT plus end trac…

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-GcAMP5

   Plasmid

   #126569

   Purpose

   Multisite gateway middle entry clone containing zebrafish calcium reporter GcAMP5. Parton lab clone ANR.

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   GcAMP5
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTTH+bromo5

   Plasmid

   #157803

   Purpose

   Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   bromo5
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103 IQGAP1 WW domain

   Plasmid

   #104279

   Purpose

   Bacterial expression of WW domain from IQGAP1

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   IQGAP1 WW domain (IQGAP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX Socs3-SH2

   Plasmid

   #46506

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Socs3 (SOCS3 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 22-142

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179430

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. cmv-sfGFP-GB1-scAB

    Plasmid

    #185794

    Purpose

    scFv GFP compnent for SunRISER

    Depositor

    Robin Lee

    Article

    Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

    Insert

    sfGFP-GB1-scAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Socs1-SH2

    Plasmid

    #46504

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs1 (SOCS1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-166

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3962 pcDNA3-IRF3-FL

    Plasmid

    #22860

    Depositor

    Peter Howley

    Article

    Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEZYHb

    Plasmid

    #18669

    Purpose

    pKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteria

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    hemoglobin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPOP-V5 (BACK))

    Plasmid

    #169722

    Purpose

    Mammalian Expression of mouse SPOP Y353E

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Y353E

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPOP-V5 (BTB)

    Plasmid

    #169721

    Purpose

    Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPTn2P-PTK

    Plasmid

    #85604

    Purpose

    Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Passport transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEW8

    Plasmid

    #84238

    Purpose

    Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

    Depositor

    David Weinberg

    Article

    DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

    Insert

    HA-mRuby-P2A-eGFP
    

    Use

    Tags

    HA-mRuby and eGFP

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBFv-nosP-Cas9

    Plasmid

    #138402

    Purpose

    Expresses Cas9 in the germline of Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    Cas9-NLS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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