169,492 results

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pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-(F30-2xPepper)10

Plasmid

#129401

Purpose

mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

mCherry-(F30-2xPepper)10

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_eGFP

Plasmid

#26822

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-eGFP-3'UTR

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5B-Flag-Smurf2 wt

Plasmid

#11746

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-mStayGold

Plasmid

#227250

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-TEX264-GFP

Plasmid

#201925

Purpose

For lentiviral transduction of TEX264-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Lentiviral

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLSB-HYG

Plasmid

#166699

Purpose

Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.

Depositor

Robin Allshire

Article

Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

Inserts

tRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe

Expression

Yeast

Promoter

adh15 promoter and tRNA promoter

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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ZCCHC6

Plasmid

#156140

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZCCHC6 (TUT7 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mhYFP

Plasmid

#186533

Purpose

Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/38.1R]

Plasmid

#114492

Purpose

Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

dual CMV

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-BHLHE40-IRES-ZsGreen1

Plasmid

#153061

Purpose

Lentiviral vector expressing human BHLHE40

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJT305 Pa01 Lenti pEF-attB LSR GFP

Plasmid

#184948

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hAAVS1 1R TALEN

Plasmid

#35432

Purpose

TALEN designed to target the human AAVS1 locus

Depositor

Feng Zhang

Article

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

hAAVS1 1R TALEN

Expression

Mammalian

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-Pink1

Plasmid

#101874

Purpose

Transient expression of Pink1-YFP in mammalian cells

Depositor

Richard Youle

Article

Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

Insert

PTEN induced putative kinase 1 (PINK1 Human)

Tags

fluorescent tag EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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tdKatushka2-VSVG-16

Plasmid

#56052

Purpose

Localization: Golgi/ER Transport, Excitation: 586, Emission: 632

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

VSVG

Tags

tdKatushka2

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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sFlt1-i13 V5

Plasmid

#86044

Purpose

human Soluble Flt1 isoform i13 (terminates in intron 13)

Depositor

Christie Thomas

Article

Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

Insert

human soluble Flt1 isoform i13 (FLT1 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

isoform i13 (terminates in intron 13)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry

Plasmid

#92202

Purpose

Fluorescent protein reporter construct

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphasS-RLuc8

Plasmid

#140980

Purpose

Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphasS-RLuc8

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV IRES Luciferase

Plasmid

#18760

Depositor

Scott Lowe

Type

Empty backbone

Use

Luciferase and Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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