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Framework for in vivo T cell screens.
Milling LE, Markson SC, Tjokrosurjo Q, Derosia NM, Streeter ISL, Hickok GH, Lemmen AM, Nguyen TH, Prathima P, Fithian W, Schwartz MA, Hacohen N, Doench JG, LaFleur MW, Sharpe AH
J Exp Med. 2024 Apr 1;221(4):e20230699. doi: 10.1084/jem.20230699. Epub 2024 Feb 27. PubMed Article

Plasmids from Article

ID Plasmid Purpose
184859pRDA_526Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.
184924pLX_305_OvalbuminLentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

Antibodies from Article