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(Plasmid #64928)


Item Catalog # Description Quantity Price (USD)
Plasmid 64928 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 7320
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • Mutation
    4EBP1 domain contains T37A and T46A mutations.
  • GenBank ID
  • Entrez Gene
    EIF4EBP1 (a.k.a. 4E-BP1, 4EBP1, BP-1, PHAS-I)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Cerulean (N terminal on insert)
    • YPet (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-TORCAR(T/A) was a gift from Jin Zhang (Addgene plasmid # 64928 ; ; RRID:Addgene_64928)
  • For your References section:

    Dynamic Visualization of mTORC1 Activity in Living Cells. Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J. Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 10.1016/j.celrep.2015.02.031. 10.1016/j.celrep.2015.02.031 PubMed 25772363