166,041 results

1. Myr.PKCzeta.FLAG

   Plasmid

   #10802

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Tags

   FLAG and Myr

   Expression

   Mammalian

   Available Since

   July 26, 2006

   Availability

   Academic Institutions and Nonprofits only

2. CROP-seq-opti

   Plasmid

   #106280

   Purpose

   A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV/SB10

   Plasmid

   #24551

   Depositor

   Perry Hackett

   Article

   Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

   Insert

   Sleeping Beauty
   

   Expression

   Bacterial

   Available Since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. GABBR1-DuET

   Plasmid

   #213247

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GABBR1 (GABBR1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-DIO-CaMKII-TVA-P2A-N2cG

   Plasmid

   #172362

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Ef1a-SunTag-RPS7-PP7-WPRE

   Plasmid

   #198338

   Purpose

   PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   GCN4x24-RPS7-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops and SunTag

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAPM-D4 miR30-MPHOSPH8 ts2

   Plasmid

   #115875

   Purpose

   MPHOSPH8 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   MPHOSPH8 shRNA (MPHOSPH8 Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH727-CEN-RLuc/staCFLuc

   Plasmid

   #38211

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The last three codons of the full-length Firefly …

   Promoter

   ADH1 and TDH3 (=GPD)

   Available Since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKMYT1

   Plasmid

   #39061

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PKMYT1 (PKMYT1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. v3em-Cterm-PE2max-∆RNaseH-dualU6

    Plasmid

    #198735

    Purpose

    AAV genome encoding C-terminal PE2max and U6 expression cassettes

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    NpuC-CtermPE2max∆RNaseH
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-hMLN

    Plasmid

    #27079

    Purpose

    Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    C-MYC, LIN28, NANOG (MYC Human)
    

    Expression

    Mammalian

    Available Since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS/U6 radixin siRNA

    Plasmid

    #8947

    Depositor

    Philip Hinds

    Article

    Yang et al Mol Cell. 2003 May . 11(5):1163-76.

    Insert

    radixin siRNA (RDX Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pLenti X2 Neo empty (w36-1)

    Plasmid

    #17478

    Purpose

    3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Neo 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF2973

    Plasmid

    #144449

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPARD (PPARD Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid

    #200676

    Purpose

    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mPA-GFP-Caveolin-C-10

    Plasmid

    #57123

    Purpose

    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Caveolin (CAV1 Human)
    

    Tags

    mPA-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

    Plasmid

    #91718

    Purpose

    AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

    Insert

    mEGFP-P2A-paAIP2
    

    Use

    AAV

    Promoter

    CaMKII 1.3

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. KA1396_pGL4-UBCmin-luc2/Klf4-E'

    Plasmid

    #124187

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV_EF1a_CDK4(R24C)-P2A-Hygro_Barcode

    Plasmid

    #170222

    Purpose

    Barcoded lentiviral vector to express CDK4 (R24C) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Lentiviral

    Mutation

    R24C

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. KA2626_pGL4-UBCmin-luc2/Klf2-D'

    Plasmid

    #124199

    Purpose

    Luciferase reporter driven by the Klf2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf2 enhancer
    

    Use

    Luciferase

    Promoter

    UBCmin

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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