161,851 results

1. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Thomas Westbrook, Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-TLR4-YFP

   Plasmid

   #13018

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 4 (TLR4 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNF02-mJuniper

   Plasmid

   #219398

   Purpose

   Constitutive expression of mJuniper in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mJuniper
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proDp

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX551

   Plasmid

   #60957

   Purpose

   pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Hyg-mEGFP

   Plasmid

   #191847

   Purpose

   Expresses the monomeric form of EGFP

   Depositor

   Bertrand Collet

   Article

   Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-GGamma9-GFP2

   Plasmid

   #140991

   Purpose

   Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma9-GFP2 (GNGT2 Human)
   

   Use

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX311 Luciferase

    Plasmid

    #117735

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-ClpX

    Plasmid

    #234733

    Purpose

    Human-codon optimized E. coli ClpX in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    ClpX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-GAL4-ERT2-VP16

    Plasmid

    #82588

    Purpose

    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

    Depositor

    Kryn Stankunas

    Article

    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

    Insert

    GAL4-ERT2-VP16
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1-TFE3

    Plasmid

    #38120

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFE3 (TFE3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1

    Plasmid

    #124651

    Purpose

    Soma-localized ChrimsonR for dense expression in excitatory neurons

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Has Service

    AAV1 and AAV9

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CamKIIa

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX_HA

    Plasmid

    #178534

    Purpose

    Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. kiCAP-AAV-MyoAAV2A

    Plasmid

    #224440

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDQTTL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Neo-Bam p53 R273H

    Plasmid

    #16439

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R273H (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R273H

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2B-mCherry

    Plasmid

    #20972

    Purpose

    H2B fused to mCherry for chromatin visualization

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Histone cluster 1, H2bb (H2BC3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2_GFP

    Plasmid

    #61764

    Purpose

    Expresses MS2 coat protein fused to GFP

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    MS2 coat protein fused to eGFP
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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