159,284 results

1. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RBM10_pLX307

   Plasmid

   #98371

   Purpose

   Lentiviral expression of RBM10

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   RBM10 (RBM10 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   E1Fa

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825)

   Plasmid

   #214862

   Purpose

   Expression of iCre(R297T) in striatal medium spiny neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   iCre(R297T)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF1662

   Plasmid

   #142368

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARID5B (ARID5B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH2.2_mCherry-MS2x12

   Plasmid

   #205521

   Purpose

   Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_V5-TWIST1

   Plasmid

   #215030

   Purpose

   Mammalian expression of human TWIST1

   Depositor

   Joanna Wysocka

   Article

   Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

   Insert

   twist family bHLH transcription factor 1 (TWIST1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mPiezo1-IRES-eGFP

   Plasmid

   #80925

   Purpose

   expresses mouse Piezo1 with GFP

   Depositor

   Ardem Patapoutian

   Article

   Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

   Insert

   mouse Piezo1 CDS (Piezo1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Peroxisomes-2

   Plasmid

   #54520

   Purpose

   Localization: Peroxisomes, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   Peroximal Targeting Signal 1 (PTS1) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-CAG-rtTA3G-IH

   Plasmid

   #214118

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   trTA3G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1636

    Plasmid

    #141856

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXC13 (HOXC13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.U6gLacZ.hSyn.flex.H2B.RFP

    Plasmid

    #170373

    Purpose

    Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-hAR-L26A,F27A

    Plasmid

    #89084

    Purpose

    mammalian expression of human androgen receptor with mutation of N-terminal FxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR FxxLF mutant (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-L26A,F27A

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pDONR221-SLC20A1_STOP

    Plasmid

    #161176

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC20A1 (SLC20A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCR8/GW:U6

    Plasmid

    #85981

    Purpose

    Golden Gate Level 0 cassette encoding the U6 promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    T. pseudonana U6 promoter
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. psubCAG-WPRE-hVEGF-D-Strept

    Plasmid

    #119227

    Purpose

    Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.

    Depositor

    Kari Alitalo

    Insert

    VEGFD (VEGFD Human)
    

    Use

    AAV

    Tags

    Strept

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. c-myc-PT3EF1a

    Plasmid

    #92046

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Xin Chen

    Article

    Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

    Insert

    C-MYC (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    July 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-P4M-SidMx2

    Plasmid

    #51472

    Purpose

    Biosensor for PtdIns4P

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Inserts

    P4M-SidM(546-647)
    P4M-SidM(546-647)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT/Flag-MSL3L1

    Plasmid

    #29761

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MSL3L1 (MSL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-hAR-W433A,L436A,F437A

    Plasmid

    #89086

    Purpose

    mammalian expression of human androgen receptor with mutations of N-terminal WxxLF motif

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    hAR WxxLF mutant (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hAR-W433A,L436A,F437A

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1 tTA UQCRC2

    Plasmid

    #177978

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to UQCRC2 sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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