Addgene

157,653 results

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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Use

Tags

anti-mouse nanobody

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

TetOx13-CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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LCV2 cGAS KO

Plasmid

#217444

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human cGAS

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting cGAS (CGAS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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VSV.G

Plasmid

#14888

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

VSV-G Envelope

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_KLF4

Plasmid

#26815

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Sep 29. ():.

Insert

5'UTR-KLF4-3'UTR (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Starts from second ATG

Promoter

Available since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pGFP-EB1

Plasmid

#17234

Depositor

Lynne Cassimeris

Article

Piehl et al Mol Biol Cell. 2003 Mar . 14(3):916-25.

Insert

EB1 (MAPRE1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2008

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#71814

Purpose

Expresses high specificity SpCas9. Px330-like plasmid.

Depositor

Feng Zhang

Article

Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

Has Service

Cloning Grade DNA

Insert

enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHIV-Luc-ZsGreen

Plasmid

#39196

Depositor

Bryan Welm

Inserts

Firefly Luciferase Luc2P
ZsGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha and EF1-alpha

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pXN21-Dest

Plasmid

#105086

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

NubG

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FKBP12-Gag(HIV)

Plasmid

#138476

Purpose

Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

HIV Gag

Use

Tags

FRBP12 and Lynn Myristolation site

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-Puro

Plasmid

#207722

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

Plasmid

#223273

Purpose

Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-tdT

Plasmid

#59171

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV LIC cloning vector (1S)

Plasmid

#29659

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

6xHis, SUMO, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-SF3B1-WT

Plasmid

#82576

Purpose

Expresses a codon-optimized ORF of human SF3B1 (wild-type)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-WT (SF3B1 Human)

Use

Tags

FLAG peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mRFP

Plasmid

#67145

Purpose

Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mRFP (ADRB2 Human, Discosoma sp.)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GPD

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMP31-1

Plasmid

#45905

Depositor

Harmit Malik

Article

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Insert

mitochondrial antiviral signaling protein (MAVS Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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mApple-Alpha-V-Integrin-25

Plasmid

#54866

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

Alpha-V Integrin (ITGAV Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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