165,666 results

1. pFUW-tetO-Relb

   Plasmid

   #139810

   Purpose

   doxycycline-inducible expression of mouse Relb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Relb (Relb Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. myr-palm-mCherry2

   Plasmid

   #197910

   Purpose

   Monomer expression vector with plasma membrane localization signal

   Depositor

   Salvatore Chiantia

   Article

   Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

   Insert

   myristoylated-palmitoylated-mCherry2
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

   Plasmid

   #86885

   Purpose

   C-terminal part of the split-BioID

   Depositor

   Mathieu Bollen

   Article

   Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

   Insert

   BirA* (AA141-321)- HA- NIPP1]
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   BirA Amino Acids 141-321

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-LSSA12

   Plasmid

   #186545

   Purpose

   LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSA12
   

   Expression

   Mammalian

   Mutation

   hfYFP-G65S/Y203I/Q204E/E222D/R223F

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.84(hRlucCP/Puro)

   Plasmid

   #212935

   Purpose

   RapidResponse(TM) Renilla luciferase vector for investigating regions controlling transcription

   Depositor

   Pete Stecha

   Type

   Empty backbone

   Use

   Luciferase

   Mutation

   WT

   Available Since

   Jan. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLenti_EF1a_Flag_EGFP

   Plasmid

   #183117

   Purpose

   Expresses flag-tagged EGFP in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

   Plasmid

   #86886

   Purpose

   N-terminal part of the split-BioID

   Depositor

   Mathieu Bollen

   Article

   Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

   Insert

   PP1g - FLAG - BirA (AA2-140)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   BirA Amino Acids 2-140

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB1deltaC-mCherry

   Plasmid

   #80603

   Purpose

   Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

   Depositor

   Torsten Wittmann

   Article

   Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Truncated C-terminus; EB1 coding sequence ends at…

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. BACE2_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176942

   Purpose

   Mammalian expression vector encoding BACE2 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   BACE2 (BACE2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0591

    Plasmid

    #142654

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO4 (FOXO4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-GluA1-scFV-Halo

    Plasmid

    #230065

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOpen-SfiIR (cDNA)

    Plasmid

    #165528

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    SfiI (used to create cDNA libraries)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLX302-MKP5-V5 puro

    Plasmid

    #87770

    Purpose

    Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-rxRFP

    Plasmid

    #67830

    Purpose

    Redox-Sensitive Red Fluorescent Protein

    Depositor

    Huiwang Ai

    Article

    Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

    Insert

    Redox-Sensitive Red Fluorescent Protein
    

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR223.1_mmDdx58 (closed)

    Plasmid

    #160107

    Purpose

    Expresses murine Ddx58 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ddx58 (Rigi Mouse)
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-MBP-TEV

    Plasmid

    #105771

    Purpose

    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    MBP-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTM

    Plasmid

    #162783

    Purpose

    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Type

    Empty backbone

    Tags

    10xHis, c-myc, and hTPA leader

    Expression

    Mammalian

    Promoter

    CHO Elongation factor 1A

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Integrin β5-GFP

    Plasmid

    #205090

    Purpose

    Express GFP-tagged human integrin β5 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 5 (ITGB5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

    Plasmid

    #203541

    Purpose

    gRNA expression in astrocytes

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-GFP, 2x U6-RNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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