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Flag-HA-OTUD5
(Plasmid #22610)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22610 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro
  • Backbone manufacturer
    Harper_Lab
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OTUD5
  • Alt name
    OTU domain containing 5
  • Alt name
    DKFZp761A052, DUBA, MGC104871
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_017602
  • Entrez Gene
    OTUD5 (a.k.a. DUBA)
  • Promoter CMV/TO
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • FLAG (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gift from Vishva Dixit
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-HA-OTUD5 was a gift from Wade Harper (Addgene plasmid # 22610 ; http://n2t.net/addgene:22610 ; RRID:Addgene_22610)
  • For your References section:

    Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732