159,284 results

1. pGS-21a his6-GST-WTPhi29DNAP

   Plasmid

   #166863

   Purpose

   To produce a dual affinity-tagged Phi29 DNA polymerase

   Depositor

   Stephen Quake, Paul Blainey

   Article

   Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

   Insert

   Phi29 Polymerase codon-optimized for overexpression in E. coli
   

   Use

   Tags

   GST and his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTagBFP2-ER-5

   Plasmid

   #55294

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   ER (CALR Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TFORF3247

   Plasmid

   #144723

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF Con/Fon DREADD Gq-mCherry

   Plasmid

   #183532

   Purpose

   INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

   Depositor

   Karl Deisseroth

   Insert

   DREADD Gq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   nEF

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-FGFR3

   Plasmid

   #116742

   Purpose

   Lentiviral expression of FGFR3

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FGFR3 (FGFR3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-PinkyCaMP

   Plasmid

   #232856

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under hSyn promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

   Plasmid

   #196223

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST53-LRRK2-G2019S

   Plasmid

   #25045

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Glycine 2019 mutated to Serine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. #2.4 post-G-SynapShot-mScarlet

   Plasmid

   #214720

   Purpose

   Expresses green #2.4 post-SynapShot and mScarlet

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #2.4 post-G-SynapShot
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-BRD4

    Plasmid

    #65378

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    R1232W

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIP-SFFV-mtagBFP-2A RIGI WT

    Plasmid

    #167289

    Purpose

    Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_pMBP-EGFP-caax

    Plasmid

    #190155

    Purpose

    Expresses membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    EGFP-caax
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MBP

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL97

    Plasmid

    #78576

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ermEp* promoter from S. erythraea

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMDIAI

    Plasmid

    #51655

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    apramycin antibiotic resistance gene
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTet_NADP-Snifit-cyto

    Plasmid

    #113917

    Purpose

    Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TetO2

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBa.Halo-LDLR

    Plasmid

    #162717

    Purpose

    Mammalian expression of human LDLR

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    LDLR (LDLR Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    David Root, William Hahn, John Doench

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJL-TRBO

    Plasmid

    #80082

    Purpose

    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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