166,201 results

1. TK4-EGFP

   Plasmid

   #239047

   Purpose

   PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

   Depositor

   Marius Wernig

   Inserts

   EGFP
   puroR
   

   Expression

   Mammalian

   Promoter

   CAG and EF1a

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV-NAT-FLAG-CIP2A FL (sg2R)

   Plasmid

   #222624

   Purpose

   Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF-1-alpha promoter

   Available Since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. TFORF3135

   Plasmid

   #144611

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NOTCH2 (NOTCH2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEmerald-LASP1-N-10

   Plasmid

   #54142

   Purpose

   Localization: Focal Adhesions, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   NM_006148 (LASP1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   A209V

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pDEST47-ARL2-GFP

   Plasmid

   #67396

   Purpose

   Mammalian expression of hArl2 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL2 (ARL2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. mOPN-pcDNA

   Plasmid

   #106447

   Purpose

   Mammalian expression of murine osteopontin

   Depositor

   Georg Weber

   Article

   Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

   Insert

   osteopontin (Spp1 Mouse)
   

   Expression

   Mammalian

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCSG53-DsbC

   Plasmid

   #186623

   Purpose

   E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

   Depositor

   Alexei Savchenko

   Article

   Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

   Type

   Empty backbone

   Tags

   DsbC-6His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

   Plasmid

   #197061

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Inserts

   Encapsulin
   Adaptor Protein
   

   Use

   CRISPR

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGD-p19

   Plasmid

   #196326

   Purpose

   35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   tomato bushy stunt virus p19
   

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-HA-14-3-3 ϵ

    Plasmid

    #48797

    Purpose

    expresses human myc-14-3-3ϵ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAE 14-3-3 ϵ (YWHAE Human)
    

    Tags

    haemagglutinin

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

12. DPYD-FLAG

    Plasmid

    #61616

    Purpose

    The human DPYD was used to study the effect of DPYD expression on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    Dihydropyrimidine dehydrogenase (DPYD Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-PLK1-N-16

    Plasmid

    #55119

    Purpose

    Localization: Centrosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    PLK1 (PLK1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC_N_STA5B

    Plasmid

    #167800

    Purpose

    Expresses STA5B in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    STA5B (STAT5B Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225701

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral

    Tags

    3xAID HA and T2A-mTurquoise

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SGLT1(GFP)-MAP17(nb)

    Plasmid

    #216210

    Purpose

    for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

    Depositor

    Lei Chen

    Article

    Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

    Insert

    SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD120

    Plasmid

    #119879

    Purpose

    Constitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pConstitutive_Che9c60 and Che9c61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Promoter

    tetON

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. GANLS-DEVD-BNES

    Plasmid

    #50835

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddRFP B
    

    Tags

    NES sequence LALKLAGLDIGS and triplicated NLS seq…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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