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p40PX-EGFP
(Plasmid #19010)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19010 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p40phox
  • Alt name
    NADPH oxidase subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    444
  • Mutation
    aa1-148 (PX domains)
  • Entrez Gene
    NCF4 (a.k.a. CGD3, NCF, P40PHOX, SH3PXD4)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p40PX-EGFP was a gift from Michael Yaffe (Addgene plasmid # 19010 ; http://n2t.net/addgene:19010 ; RRID:Addgene_19010)
  • For your References section:

    The PX domains of p47phox and p40phox bind to lipid products of PI(3)K. Kanai F, Liu H, Field SJ, Akbary H, Matsuo T, Brown GE, Cantley LC, Yaffe MB. Nat Cell Biol. 2001 Jul . 3(7):675-8. 10.1038/35083070 PubMed 11433300