159,333 results

1. PM-FRB-mRFP-T2A-FKBP-5-ptase

   Plasmid

   #40896

   Depositor

   Peter Varnai

   Article

   Toth et al J Cell Sci. 2012 May 1;125(Pt 9):2185-97

   Insert

   PM-FRB-mRFP-T2A-FKBP-5-ptase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. piggyBAC-5xTAPE-1-BlastR

   Plasmid

   #175808

   Purpose

   Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Insert

   5xTAPE-1 construct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACBSR-hyg

   Plasmid

   #87830

   Purpose

   Expresses lambda-red system with hyg selection marker

   Depositor

   Pep Charusanti

   Article

   Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

   Insert

   hyg
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   None

   Promoter

   bla

   Available since

   Aug. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. #1.4 pre-R-SynapShot-TagBFP

   Plasmid

   #214719

   Purpose

   Expresses red #1.4 pre-SynapShot and TagBFP

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #1.4 pre-R-SynapShot
   

   Use

   AAV

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE17

   Plasmid

   #90359

   Purpose

   NFAT - PKC/Ca++ gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFAT

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-Hylight-T152E

   Plasmid

   #193448

   Purpose

   A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

   Depositor

   Richard Goodman

   Article

   Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Binding-Dead Hylight
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

   Promoter

   CMV

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGM1190

   Plasmid

   #69994

   Purpose

   OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aac
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PPRE-pNL1.3

   Plasmid

   #84394

   Purpose

   Secreted Luciferase reporter for PPARgamma activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

   Insert

   PPARG response element (PPARG Human)
   

   Use

   Tags

   secNluc

   Expression

   Mammalian

   Mutation

   Promoter

   PPRE

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. nAChR beta2 WT

   Plasmid

   #24272

   Depositor

   Henry Lester

   Article

   Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

   Insert

   nAChR beta2 WT (Chrnb2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mEGFP-N1

    Plasmid

    #54767

    Purpose

    Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. TFORF1653

    Plasmid

    #143533

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

    Plasmid

    #197044

    Purpose

    In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

    Depositor

    Liqun Luo

    Article

    Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

    Inserts

    pCAG-FSF-LSL
    SP-HA-HRPtm
    WPRE-bGHpolyA
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. eGFP-luciferase-C1

    Plasmid

    #163523

    Purpose

    Expresses both eGFP and firefly luciferase in mammalian cells

    Depositor

    Thomas Weber

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    eGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. nAChR alpha4 WT

    Plasmid

    #24271

    Depositor

    Henry Lester

    Article

    Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

    Insert

    nAChR alpha4 WT (Chrna4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

    Plasmid

    #218793

    Purpose

    AAV reporter genome plasmid

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    NLSf-mScarletP2A-hLuc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC0056 - LwCas13a-msfGFP-NES

    Plasmid

    #105815

    Purpose

    Expresses active LwaCas13a-NES for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NES and msfGFP-NES-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-HA-USP48

    Plasmid

    #22585

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP48 (USP48 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMLS7-egfp

    Plasmid

    #112917

    Purpose

    Fluorescence microscopy

    Depositor

    Miguel Valvano

    Article

    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PS7 promoter and Ps7

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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