164,223 results

1. pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132397

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1058A

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIN4-EF2-ABCG2-IRES-Neo

   Plasmid

   #25983

   Depositor

   Ren-he Xu

   Article

   Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

   Insert

   ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
   

   Use

   Lentiviral

   Tags

   EF1-apha promoter and IRES-Neo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-LMP1-IRES-GFP

   Plasmid

   #220350

   Purpose

   Expresses EBV latent gene LMP1 in mammalian cells

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLSSmOrange-mKate2 caspase-3 biosensor

   Plasmid

   #37132

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

   Insert

   LSSmOrange-DEVD-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 MDM2 WT

   Plasmid

   #16233

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL99

   Plasmid

   #78577

   Purpose

   Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

   Depositor

   Xuming Mao

   Article

   Sun et al Annals of Microbiology Dec 2012, Volume

   Type

   Empty backbone

   Use

   Streptomyces expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   inducible PnitA-NitR system

   Available Since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIV643

   Plasmid

   #226182

   Purpose

   Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   uidA marker flanked with targeting regions to KU70 gene in K.phaffii
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TEF1p

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

   Plasmid

   #114070

   Purpose

   T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP(N3)-PP1beta

   Plasmid

   #44223

   Depositor

   Angus Lamond, Laura Trinkle-Mulcahy

   Article

   Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

   Insert

   PP1B (PPP1CB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. epi-BE4max

    Plasmid

    #135975

    Purpose

    Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209722

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC1 Grx1-roCherry-mito

    Plasmid

    #141069

    Purpose

    Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

    Depositor

    Dmitry Bilan

    Article

    Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

    Insert

    Grx1-roCherry
    

    Use

    Tags

    dMTS

    Expression

    Mammalian

    Mutation

    Changed Alanine 150 to Cysteine, inserted Threoni…

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pV1093

    Plasmid

    #111428

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-MRLC1

    Plasmid

    #35680

    Depositor

    Tom Egelhoff

    Article

    Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAAV FLEX Synaptophysin GFP

    Plasmid

    #137188

    Purpose

    Cre-dependent expression of synaptophysin-GFP

    Depositor

    Matthew Nolan

    Article

    Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

    Insert

    Synaptophysin GFP (Syp Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AICSDP-7:SEC61B-mEGFP

    Plasmid

    #87426

    Purpose

    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

    Insert

    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    March 15, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. AAV-FLEX-FLIM-AKAR

    Plasmid

    #60445

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-FLAG-Ku80

    Plasmid

    #46958

    Purpose

    Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku80 (XRCC5 Human)
    

    Use

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCH1/RAD51o

    Plasmid

    #102562

    Purpose

    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

    Depositor

    Maria Spies

    Article

    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

    Inserts

    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli Expression

    Promoter

    T7

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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