166,201 results

1. pET-Duet1_6xHis-TEV-eGFP-P62

   Plasmid

   #190929

   Purpose

   Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   P62 (SQSTM1 Human)
   

   Tags

   EGFP

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-OPTN

   Plasmid

   #171424

   Purpose

   For the purifcation of OPTN protein

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   OPTN (OPTN Human)
   

   Expression

   Bacterial

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRA108

   Plasmid

   #209341

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLPCX-Cx43-IRES-GFP

   Plasmid

   #65433

   Purpose

   Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

   Depositor

   Trond Aasen

   Article

   Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

   Insert

   Cx43 (GJA1 Human)
   

   Use

   Retroviral

   Tags

   IRES-GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFNMB2-msfGFP

   Plasmid

   #113192

   Purpose

   Mobilizable plasmid for tetracycline inducible gene expression in Francisella novicida

   Depositor

   Marek Basler

   Article

   Brodmann et al Front Cell Infect Microbiol. 2018 Aug 31

   Insert

   msfGFP
   

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-P-CAG-mCh

   Plasmid

   #80492

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   mCherry
   

   Use

   Donor vector (human)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-L3L2

   Plasmid

   #114019

   Purpose

   Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

   Depositor

   Christopher Grefen

   Insert

   ccdB gene, Chloramphenicol resistance gene
   

   Use

   Gateway

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGPI-SceI

    Plasmid

    #32060

    Purpose

    Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

    Depositor

    Miguel Valvano

    Article

    Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

    Type

    Empty backbone

    Use

    Mutagenesis plasmid

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-L1L4

    Plasmid

    #114018

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Tol2

    Plasmid

    #31823

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Insert

    Tol2 Transposase
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-TID-HA3-KanMX6

    Plasmid

    #227980

    Purpose

    To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PP7_mCherry

    Plasmid

    #61763

    Purpose

    Expresses PP7 coat protein fused to mCherry

    Depositor

    Daniel Larson

    Article

    Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

    Insert

    PP7 coat protein fused to mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Ubc promoter

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP

    Plasmid

    #176015

    Purpose

    Mammalian expression of EGFP. Parton lab clone DFS

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    EGFP
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0195

    Plasmid

    #142554

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RFX7 (RFX7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBbB2k-GFP

    Plasmid

    #35345

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST47-ARL3-GFP

    Plasmid

    #67397

    Purpose

    Mammalian expression of hArl3 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Ch-gelsolN

    Plasmid

    #37262

    Depositor

    Ikuo Wada

    Article

    Nagaya et al J Cell Biol. 2008 Jan 14;180(1):129-43.

    Insert

    gelsolin (GSN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deleted amino acids 380-731

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pTetOne-mCherry-cNLS

    Plasmid

    #138527

    Purpose

    Inducible expression of mCherry with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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