159,333 results

1. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PH-Btk-GFP

   Plasmid

   #51463

   Purpose

   Is a biosensor for PIP3

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   BTK (aa 1–177) (BTK Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b PKA Cat

   Plasmid

   #14921

   Depositor

   Susan Taylor

   Article

   Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

   Insert

   PKA catalytic subunit alpha (Prkaca Mouse)
   

   Use

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTMB304_ZFcharm_Kv1_Prnp_SCR

   Plasmid

   #220842

   Purpose

   AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv1
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-Flag-BRD4-WT

   Plasmid

   #90331

   Purpose

   expresses Wild type BRD4

   Depositor

   Kornelia Polyak

   Article

   Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. eGFP-hCOL1A1

   Plasmid

   #140110

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag, K20…

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

   Plasmid

   #203350

   Purpose

   Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Molly Stevens, Christopher Grigsby

   Insert

   Nano Luciferase
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEvol-NnSULT1C1-cysDNCQ

   Plasmid

   #188983

   Purpose

   biosynthesize sulfotyrosine in E. coli.

   Depositor

   Han Xiao

   Article

   Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

   Inserts

   NnSULT1C1
   cysD
   cysN
   cysC
   cysQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0043-PspCas13b crRNA backbone

    Plasmid

    #103854

    Purpose

    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6-BbsI-BbsI-PspCas13b DR-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SV40 1: pBSSVD2005

    Plasmid

    #21826

    Depositor

    David Ron

    Insert

    SV40 Large T (SV40gp6 simian virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    likely expresses aa109-708 of the large T antigen

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. HDAC3 Flag

    Plasmid

    #13819

    Purpose

    Mammalian expression of human histone deacetylase 3 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC3 (HDAC3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. EB3-mNeonGreen

    Plasmid

    #191761

    Purpose

    Expression of mNeonGreen fused to EB3

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    EB3-mNeonGreen (MAPRE3 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FTL

    Plasmid

    #100750

    Purpose

    Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    FTL (FTL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. shYAP1 # 1

    Plasmid

    #42540

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1a-DIO-hM4D(Gi)-mCherry

    Plasmid

    #50461

    Purpose

    Double floxed Gi-coupled hM4D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-mCherry (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-CAG-mRuby3-Gal8-P2A-Zeo

    Plasmid

    #150815

    Purpose

    Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

    Insert

    mRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)
    

    Use

    Tags

    Gal8 is fused to the c-terminus of mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

    Plasmid

    #141385

    Purpose

    Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-E (E SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pXL001-TQM IL13 CAR

    Plasmid

    #154054

    Purpose

    Lentiviral expression of TQM IL13 CAR for CAR-T therapy

    Depositor

    Xiaojun Lian

    Article

    Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

    Insert

    TQM IL13 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge