166,201 results

1. pLV-sgCDKN1B#2 BFP

   Plasmid

   #60905

   Purpose

   A lentiviral plasmid that encodes both a sgRNA targeting CDKN1B and BFP

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-CAG::Nucbow

   Plasmid

   #158992

   Purpose

   Multicolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Nucbow
   

   Tags

   All FPs are fused to H2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMU-ACTFr

   Plasmid

   #61191

   Purpose

   Fluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtFimbrin1-ABD
   

   Tags

   mCherry

   Expression

   Plant

   Promoter

   AtUBQ10

   Available Since

   Feb. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. FR_HNF1B

   Plasmid

   #31101

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   wild type

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_iNEUROD1_P2A_GFP_Puro

   Plasmid

   #168803

   Purpose

   PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   NEUROD1 (NEUROD1 Synthetic, Human)
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDAI-SceI-SacB

   Plasmid

   #113635

   Purpose

   Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

   Depositor

   Miguel Valvano

   Article

   Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

   Insert

   sacB
   

   Expression

   Bacterial

   Mutation

   Please see Depositor Comments

   Promoter

   sacB promoter

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FU-mILa-CRW

   Plasmid

   #84556

   Purpose

   lentiviral expression of mouse IL1a and mRFP

   Depositor

   Li Xin

   Article

   Zhang et al Mol Cell. 2016 Sep 15;63(6):976-89. doi:

   Insert

   IL1a (Il1a Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPI4-Strep-SNAP-PD-1

   Plasmid

   #223569

   Purpose

   Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-1 (PDCD1 Human)
   

   Tags

   Twin-Strep, SNAP

   Expression

   Mammalian

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRA104

   Plasmid

   #209337

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid

    #117274

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV and IRES

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC1749

    Plasmid

    #127983

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVXCMV100-mNeonGreen-PBD

    Plasmid

    #241366

    Purpose

    Lentiviral expression of mNeonGreen-tagged PAK1 p21-binding domain (PBD)

    Depositor

    Gaudenz Danuser

    Article

    Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

    Insert

    Pak1 fragment (PAK1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Mutation

    aa 57-141

    Promoter

    Truncated CMV promoter

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-HA_WT POLI

    Plasmid

    #201440

    Purpose

    Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_FLAG-Mx1201 PylRS

    Plasmid

    #154764

    Purpose

    plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pepper-fused sgRNA

    Plasmid

    #217520

    Purpose

    Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

    Depositor

    Xing Li

    Article

    Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

    Insert

    Pepper-fused sgRNA for spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid

    #122964

    Purpose

    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    human Chibby1 (CBY1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    FoxJ1 and human FoxJ1

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-BLK

    Plasmid

    #23940

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    BLK (BLK Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only