166,201 results

1. pKLV-EF1a-BFP-W

   Plasmid

   #208757

   Purpose

   Lentiviral vector expressing BFP

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mEGFP-N1-MST2

   Plasmid

   #166475

   Purpose

   Expresses fusion of mEGFP and MST2

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, MST2 (STK3 Human)
   

   Expression

   Mammalian

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pB80-2xFKBP-L-mCherry-CAAX

   Plasmid

   #193713

   Purpose

   Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   2xFKBP-L-mCherry-CAAX
   

   Tags

   2xFKBP-mCherry

   Expression

   Mammalian

   Mutation

   mCherry: Met1 Deleted

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EIF2AK1 gRNA (BRDN0001162524)

   Plasmid

   #77050

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK1 (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCIN BRCA2 WT

   Plasmid

   #16245

   Depositor

   Mien-Chie Hung

   Article

   Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

   Insert

   BRCA2 (BRCA2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-EPHA3

   Plasmid

   #116733

   Purpose

   Lentiviral expression of EPHA3

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EPHA3 (EPHA3 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHL-sec

   Plasmid

   #99845

   Purpose

   Mammalian expression with secretion signal sequence

   Depositor

   Edith Yvonne Jones

   Article

   Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   ephrin b2 (EFNB2 Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmr101A-cPr-mCherry (ErmR)

   Plasmid

   #177206

   Purpose

   Plasmid with p15A origin for constitutive mCherry expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   Synthetic constitutive promoter

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF-hSEMA4C-Fc

    Plasmid

    #74449

    Purpose

    Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

    Depositor

    Roland Friedel

    Article

    Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

    Insert

    human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)
    

    Tags

    human Fc tag

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO.1 - TRC control

    Plasmid

    #10879

    Purpose

    Negative control shRNA vector containing non-hairpin insert.

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmPB3-VSV

    Plasmid

    #68039

    Purpose

    Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Plexin-B3 (Plxnb3 Mouse)
    

    Tags

    VSV

    Expression

    Mammalian

    Available Since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEasyG3_mic

    Plasmid

    #184920

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-mCherry-KATNA1

    Plasmid

    #180650

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ttr:mRFP1

    Plasmid

    #32607

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Kwon et al Genesis. 2009 Jul;47(7):447-55.

    Insert

    TTR:mRFP
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

    Plasmid

    #193717

    Purpose

    Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Synthetic, Human)
    

    Tags

    2xmNeongreen-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF1825

    Plasmid

    #143322

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARNT (ARNT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lvl0 nluc CDS

    Plasmid

    #153404

    Purpose

    Nanoluciferase coding sequence

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    nanoluciferase CDS
    

    Expression

    Plant

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge