164,223 results

1. H3K9me3Probe_FullLength

   Plasmid

   #186463

   Purpose

   Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

   Depositor

   Chongli Yuan

   Article

   Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

   Insert

   dimeric CDY1 (CDY1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBR BsmAIM Kan + p15A BsaIR Amp

   Plasmid

   #206208

   Purpose

   Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   BsamAIM and BsaIR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. dTAG-IRF8

   Plasmid

   #190620

   Purpose

   Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation

   Depositor

   Junwei Shi

   Article

   Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

   Insert

   IRF8 (IRF8 Human)
   

   Use

   Lentiviral

   Tags

   FKBP12_G36V

   Expression

   Mammalian

   Mutation

   synonymous mutation for sgRNA resistance for sgIR…

   Promoter

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. iRFP-H2A

   Plasmid

   #158691

   Purpose

   This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcGFP1-N1-Dsup

   Plasmid

   #90020

   Purpose

   To express GFP-tagged Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. hWIP1 FLAG

   Plasmid

   #28105

   Depositor

   Lawrence Donehower

   Article

   Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

   Insert

   WIP1 (PPM1D Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN4-EF2-ABCG2-IRES-Neo

   Plasmid

   #25983

   Depositor

   Ren-he Xu

   Article

   Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

   Insert

   ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
   

   Use

   Lentiviral

   Tags

   EF1-apha promoter and IRES-Neo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lck-mTurquoise2

   Plasmid

   #98822

   Purpose

   In vivo visualization of the plasma membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Lck
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCS-TurboID MSCV-IRES-GFP

    Plasmid

    #207957

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-Tet-ON-shCtrl (neo)

    Plasmid

    #192076

    Purpose

    Lentivirus for Dox-inducible expression of a non-targeting control shRNA

    Depositor

    Roland Friedel

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1 / Tet-responsive element

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. hWIP1 D314A FLAG

    Plasmid

    #28106

    Depositor

    Lawrence Donehower

    Article

    Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-

    Insert

    WIP1 (PPM1D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains phosphatase-dead mutation D314A

    Promoter

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

14. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CAG-M-SPOTIT2

    Plasmid

    #191489

    Purpose

    Green fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    M-SPOTIT2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDsgRNA-pgaC

    Plasmid

    #154919

    Purpose

    aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pgaC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHis-hTG2

    Plasmid

    #100719

    Purpose

    Expression of human transglutaminase 2 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

    Insert

    TGM2 (TGM2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    silent mutation Arg580 (AGG--CGT) to enhance expr…

    Promoter

    T7

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. P4-PE

    Plasmid

    #211375

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-PE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. gag-MCP-pol

    Plasmid

    #211370

    Purpose

    Packaging plasmid for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-MCP-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge