166,201 results

1. pEasyG3_nat

   Plasmid

   #184918

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyG3_mic

   Plasmid

   #184920

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-mCherry-KATNA1

   Plasmid

   #180650

   Purpose

   Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GGACAGCACUCCCUUGAAA

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ttr:mRFP1

   Plasmid

   #32607

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Kwon et al Genesis. 2009 Jul;47(7):447-55.

   Insert

   TTR:mRFP
   

   Expression

   Mammalian

   Available Since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

   Plasmid

   #193717

   Purpose

   Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Synthetic, Human)
   

   Tags

   2xmNeongreen-FRB

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1825

   Plasmid

   #143322

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ARNT (ARNT Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET19-M(BRCT)TdTwt

   Plasmid

   #207459

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   M(BRCT)TdTwt (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lvl0 nluc CDS

   Plasmid

   #153404

   Purpose

   Nanoluciferase coding sequence

   Depositor

   Naomi Nakayama

   Article

   Andreou et al bioRxiv, March 31, 2021

   Insert

   nanoluciferase CDS
   

   Expression

   Plant

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFNMB1-msfGFP

   Plasmid

   #113191

   Purpose

   Mobilizable plasmid for tetracycline inducible gene expression in Francisella novicida

   Depositor

   Marek Basler

   Article

   Brodmann et al Front Cell Infect Microbiol. 2018 Aug 31

   Insert

   msfGFP
   

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-SLC25A3_STOP

    Plasmid

    #161206

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A3 (SLC25A3 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pRnc-gGFP161

    Plasmid

    #189536

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

    Depositor

    Marc Ostermeier

    Article

    Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

    Inserts

    RNase III
    gUTR-161-GFP
    

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    J23119 and pBAD

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid

    #193720

    Purpose

    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyCatcher003-mi3

    Plasmid

    #159995

    Purpose

    Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyCatcher003-mi3
    

    Tags

    C-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJG/BETA MHC promoter

    Plasmid

    #53963

    Purpose

    transgene expression driven by BETA MHC promoter

    Depositor

    Jeffrey Robbins

    Article

    Rindt et al J Biol Chem. 1993 Mar 5;268(7):5332-8.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    beta MHC

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

15. VAMP2-pHmScarlet

    Plasmid

    #166890

    Purpose

    For vesicle exocytosis visualization using pHmScarlet

    Depositor

    Pingyong Xu

    Article

    Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

    Insert

    VAMP2 (Vamp2 Mouse)
    

    Tags

    pHmScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+) eGFP PPP1R8 G4

    Plasmid

    #129021

    Purpose

    Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+) eGFP EML2 G4

    Plasmid

    #129023

    Purpose

    Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR

    Depositor

    Jeremy Wilusz

    Article

    Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGCGFP-puro

    Plasmid

    #31266

    Depositor

    Andrew Pierce

    Insert

    GCGFP
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGGD_RT_SRDX

    Plasmid

    #136976

    Purpose

    Goldengate/Greengate module EAR-repression domain

    Depositor

    Klaus Theres

    Insert

    SRDX
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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