166,201 results

1. pLJC2-Blast-DHFR

   Plasmid

   #233893

   Purpose

   lentiviral overexpression vector for DHFR

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   DHFR (DHFR Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. BLA_CAT_K136_GPG_LOV2

   Plasmid

   #231575

   Purpose

   This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   CAT_ carrying an AsLOV2 insertion behind K136
   

   Expression

   Bacterial

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IP-EGFP-PA-BAX(lyso)

   Plasmid

   #228939

   Purpose

   Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   TMEM106B (TMEM106B Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_GST-TEV-WIPI3

   Plasmid

   #223800

   Purpose

   Expression of recombinant protein for purification

   Depositor

   James Hurley, Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   WIPI3 (WDR45B Human)
   

   Tags

   GST-TEV

   Expression

   Mammalian

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXJ-Myc-IRF8

   Plasmid

   #232668

   Purpose

   transient expresses IRF8 in mammalian cells

   Depositor

   Chen-Yang Shen

   Insert

   IRF8 (IRF8 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0442

   Plasmid

   #143026

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZFX (ZFX Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBeta-MHC-promoter DV (JDW 26)

   Plasmid

   #229835

   Purpose

   A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP13592 - pAAV-AiE0519h_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3592)

   Plasmid

   #229914

   Purpose

   AiE0519h_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416-HIS3p-TID-FKBP-HA3

   Plasmid

   #228006

   Purpose

   Expresses TurboID-FKBP fusion from HIS3 promoter; yeast BioID

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   TurboID-FKBP
   

   Tags

   HA tag

   Expression

   Yeast

   Promoter

   HIS3

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFa6-TID-FKBP-3HA-KanMX6

    Plasmid

    #228024

    Purpose

    To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICOZ-MAG[EF1α-HER1 CD28Z]

    Plasmid

    #218335

    Purpose

    Donor plasmid of MAG Transposon with HER1 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HER1 CAR mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-[EF1α-HER1 CD28Z]

    Plasmid

    #218339

    Purpose

    Lentiviral transfer plasmids for mammalian expression HER1 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HER1 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRLSIN-[EF1α-HER1 4-1BB]

    Plasmid

    #218341

    Purpose

    Lentiviral transfer plasmids for mammalian expression HER1 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HER1 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH2.156_LV-Zeo

    Plasmid

    #205554

    Purpose

    Express zeocin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Zeo
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF1897

    Plasmid

    #142285

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2C1 (NR2C1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDx_mScarlet-SYFP2

    Plasmid

    #189762

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB80-hsKIF5B(1-353)GCN4-L-GFP

    Plasmid

    #193714

    Purpose

    Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Synthetic, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-353);

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

    Plasmid

    #193715

    Purpose

    Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Synthetic, Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-353); mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid

    #193719

    Purpose

    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Synthetic, Human)
    

    Tags

    2xHA-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid

    #193721

    Purpose

    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Synthetic, Human)
    

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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