162,094 results
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CAG-GFP-IRES-CRE
Plasmid#48201Purposeretroviral vector encoding for GFP and Cre-recombinaseDepositorInsertseGFP
Cre
UseRetroviralTagsExpressionMammalianMutationPromoterCAG and linked by IRESAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-KP1
Plasmid#206504PurposeAAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoterDepositorInsertKP1 capsid
UseAAVTagsExpressionMutationPromoterp5Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-OMM-GCaMP6f
Plasmid#127874PurposeFluorescent reporter for mitochondrial outer membrane Ca2+DepositorInsertOuter membrane localized GCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
Plasmid#60231PurposeExpresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
EGFP
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA-P2A and EGFP-KASHExpressionMammalianMutationPromoterU6 and hSynAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-Kan
Plasmid#224077PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selectionDepositorInsertymScarletI3
UseTagsymScarletI3ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Rtn4a-GFP
Plasmid#61807PurposeHuman Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expressionDepositorInsertRtn4a (RTN4 Human)
UseTagsAcGFPExpressionMammalianMutationRtn4a Stop Codon removedPromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
UseTagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA
Plasmid#158972PurposeVector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neuronsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
UseTagsExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Blast)
Plasmid#80921PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV14_Fibrillarin
Plasmid#73061Purposeexpression clone for human Fibrillarin with C-terminal 3xFLAG-tagDepositorInsertFibrillarin (FBL Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_Giantin_C1
Plasmid#85048PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.22-VEGF-HRE::dLUC
Plasmid#128096PurposeHypoxia response element real-time luciferase reporterDepositorInsertHypoxia response element (x5) from VEGF promoter (VEGFA Human)
UseLuciferaseTagsdrives expression of destabilized luciferase (2CP…ExpressionMammalianMutationPromoterinserted VEGF hypoxia response elements (HRE) (x5)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-hAXL
Plasmid#124797PurposeTet-inducible AXL expression in mammalian cellsDepositorInsertAXL (AXL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
JD633
Plasmid#160393PurposeCRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloningDepositorInsertsZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA
UseBinary vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT1T2
Plasmid#50590PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT1, U626t plus, U629p, T2
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
PA-GFP-α-Tubulin
Plasmid#213900PurposeImaging of Tubulin using Photoactivatable GFPDepositorInsertPA-GFP-α-Tubulin
UseTagsPhotoactivatable GFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only
