173,280 results

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pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-UbiquitinC-C-18

Plasmid

#55149

Purpose

Localization: Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

UbiquitinC (UBA52 Human)

Tags

mCherry

Expression

Mammalian

Mutation

aa 2-76 of NP_003324.1

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pTet-IRES-EGFP

Plasmid

#64238

Purpose

Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

Depositor

Maria Lung

Article

Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

pTet

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pR I-Ab alpha BirA

Plasmid

#113638

Purpose

Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

I-Ab alpha BirA (H2-Ab1 Mouse)

Tags

BirA biotinylation signal site and fused to Fos-J…

Expression

Insect

Mutation

transmembrane and cytoplasmic domains truncated

Promoter

Metallothionein

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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mNeonGreen

Plasmid

#189976

Purpose

Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF2

Plasmid

#198578

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF2 (BALF2 Epstein-Barr virus)

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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CKAR

Plasmid

#14860

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR223-LMP2A

Plasmid

#195816

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP2A

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP2A (LMP-2A Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-RNaseHI-NES-EGFP

Plasmid

#196701

Purpose

Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

bacterial RNaseHI

Use

Lentiviral

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_mGL_PP7

Plasmid

#232440

Purpose

expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

mGreenLantern

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-LUC

Plasmid

#218630

Purpose

This plasmid contains luciferase under doxycycline inducible control.

Depositor

Valentin Cracan

Article

Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

Insert

LUC (LOC116160065 )

Use

Lentiviral, Luciferase, and Synthetic Biology

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-HPF1-His-Sumo-Flag

Plasmid

#111577

Purpose

Expresses full length HPF1 in E. coli

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

HPF1 (HPF1 Human)

Tags

6xHis-Sumo-FLAG

Expression

Bacterial

Promoter

T7

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-NLRP3

Plasmid

#73955

Purpose

Express EGFP-tagged human NLRP3 in mammalian cells

Depositor

Christian Stehlik

Article

Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

Insert

NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV iE

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

Plasmid

#174516

Purpose

Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

PylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon

Expression

Bacterial

Mutation

CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…

Promoter

GlnS and lpp

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Axin2-d2EGFP

Plasmid

#21276

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 promoter (Axin2 Mouse)

Tags

d2EGFP

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCE-BiFC-VC155

Plasmid

#22020

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VC155

Tags

HA

Expression

Worm

Mutation

Changed Alanine 206 to Lysine

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pV231-xPE

Plasmid

#225257

Purpose

Mammalian expression of xPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

xPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF4

Plasmid

#195835

Purpose

Gateway Entry Clone of Epstein-Barr virus BALF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF4 (BALF4 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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