166,201 results

1. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

   Plasmid

   #221358

   Purpose

   Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC

   Plasmid

   #221359

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC; to be used with Anti-V5 [IPI-SV5-Pk1] light chain (Plasmid 221360) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. GFP-N-WASP

   Plasmid

   #47406

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   N-WASP
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-Tol2 transposase

   Plasmid

   #158774

   Purpose

   Expression of Tol2 transposase

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   Tol2 tranposase
   

   Expression

   Mammalian

   Mutation

   Tol2 codon usage frequency well suitable for muri…

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. psfGFP-LAMP1-mCherry (pHLARE)

   Plasmid

   #164477

   Purpose

   Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

   Depositor

   Diane Barber

   Article

   Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Tags

   mCherry and superfolder GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2_EF1a_TetOn_IRES_mCherry

   Plasmid

   #204155

   Purpose

   Lentivirus backbone expressing rtTA linked to mCherry

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   Tet-On 3G
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAM5188

   Plasmid

   #120087

   Purpose

   Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

   Depositor

   Susan Golden

   Article

   Chen et al Microbiology. 2016 Dec;162(12):2029-2041

   Insert

   pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
   

   Use

   Other

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCmini-iCAP-PHP.N

    Plasmid

    #127851

    Purpose

    non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

    Insert

    Synthetic construct isolate AAV-PHP.N VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-XBP1 mNeonGreen NLS

    Plasmid

    #115968

    Purpose

    ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    X-box binding protein 1 (XBP1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    HA tag, c-myc NLS, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

    Plasmid

    #60904

    Purpose

    The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

    Plasmid

    #221360

    Purpose

    Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

    Plasmid

    #187336

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. v5 Cas9-eVLP

    Plasmid

    #228468

    Purpose

    Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–Cas9
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF1α-Cre-2A-mScarlet

    Plasmid

    #207745

    Purpose

    Expression of the Cre recombinase linked to a mScarlet fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-mScarlet
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

    Plasmid

    #108871

    Purpose

    Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

    Depositor

    Alex Schier

    Article

    Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

    Inserts

    5xU6:sgRNA
    Cas9-t2A-GFP
    

    Use

    CRISPR

    Promoter

    U6 and hsp70

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCap-101-Apr

    Plasmid

    #227507

    Purpose

    Capture plasmid with apramycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Plenti-CMV-MCS-RFP-SV-puro

    Plasmid

    #109377

    Purpose

    This plasmid expresses dsRed2 and puromycin resistance

    Depositor

    Jonathan Garlick, Behzad Gerami-Naini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBR8k-GFPuv

    Plasmid

    #106385

    Purpose

    L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge