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Tol2-lyzC-mito-Dendra2

Plasmid

#107590

Purpose

Expresses mitochondria localized Dendra2 in zebrafish neutrophils

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

mito-Dendra2

Use

Tags

mitochondira localization sequence

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-KIF1B beta-FL

Plasmid

#79108

Purpose

Mammalian expression of GFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta full length (KIF1B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
T7-CMVtrans-FFLuc-polyA

Plasmid

#101156

Purpose

Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

Depositor

Marcel Bruchez

Article

Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBI121-GFP-RmMYB108

Plasmid

#173181

Purpose

Expresses Rosa multiflora MYB108 for subcellular localization in plants

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F-BG4

Plasmid

#55756

Purpose

Expresses BG4 scFv anti-G-quadruplex antibody in bacteria

Depositor

Shankar Balasubramanian

Article

Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1

Insert

BG4

Use

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMX/cyclinB1 (17)

Plasmid

#26060

Depositor

Jonathon Pines

Article

Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

Insert

Cyclin B1 (CCNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
Lv EF1a SS18-Frb PGK Puro

Plasmid

#102811

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

SS18-Frb

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-RPA40

Plasmid

#17658

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA40 (POLR1C Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
Lv EF1a MS2-Fkbp2x 2A Hygro

Plasmid

#102807

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

MS2-Fkbp2x

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GPD

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Ef1a-fDIO-GCaMP6f

Plasmid

#128315

Purpose

Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Patricia Jensen

Article

Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

Has Service

AAV1

Insert

GCaMP6f

Use

AAV

Tags

His Tag

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIIA

Plasmid

#186825

Purpose

Expresses FLAG-CaMKIIA in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2a (Camk2a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMK391 (mAID-BSD)

Plasmid

#121192

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-BSD

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH-PIK3CA H1047R

Plasmid

#180031

Purpose

This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.

Depositor

Xin Chen

Article

Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Promoter

EF1a

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSW039

Plasmid

#140035

Purpose

KO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoter

Depositor

Peter Nixon

Article

Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

Insert

Yellow fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

cLac143

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Ubiquitin (1-76; T66V, L67N)

Plasmid

#110755

Purpose

Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Use

Tags

Expression

Bacterial

Mutation

T66V, L67N

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-ABE8e(V106W)

Plasmid

#198533

Purpose

Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-ABE8e(V106W)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGFAP-TetOn3G

Plasmid

#133728

Purpose

Plasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

TetOn3G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

pGFAP

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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