166,201 results

1. pQC membrane TdTomato IX

   Plasmid

   #37351

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   membrane TdTomato
   

   Tags

   Palmitoylation signal*

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. murine TCR OTI-2A.pMIG II

   Plasmid

   #52111

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   T2A (from Thosea asigna virus)

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCL-CellCycle

   Plasmid

   #132429

   Purpose

   Expressing Fucci cell cycle indicator in mammalian cells

   Depositor

   John M. Greally

   Article

   Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

   Insert

   hCdt, hGeminin
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HIVGKO

   Plasmid

   #112234

   Purpose

   HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

   Depositor

   Eric Verdin

   Article

   Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

   Insert

   csGFP-EF1a-mKO2
   

   Use

   Lentiviral

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST_140_LVL2 cam

   Plasmid

   #179334

   Purpose

   NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Mutation

   Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-CMV-jGCaMP8m

   Plasmid

   #162372

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mVenus C1

   Plasmid

   #27794

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus C1
   

   Expression

   Mammalian

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmScarlet-I3_C1

   Plasmid

   #189756

   Purpose

   C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXJ40 GADD34 Wt (1-674aa)

   Plasmid

   #75478

   Purpose

   mammalian expression of GADD34

   Depositor

   Shirish Shenolikar

   Article

   Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

   Insert

   GADD34 (PPP1R15A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. spNW50

    Plasmid

    #173486

    Purpose

    Expresses spNW50 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW50
    

    Tags

    Histag

    Expression

    Bacterial

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mNeonGreen-mRuby2-FRET-10

    Plasmid

    #58179

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

    Type

    Empty backbone

    Tags

    mNeonGreen and mRuby2

    Expression

    Mammalian

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDual_dsCas9_Blast

    Plasmid

    #214681

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lv EF1a dCas9-HA 2A Blast

    Plasmid

    #102812

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    dCas9-HA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A (dCas9)

    Promoter

    EF1a

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

    Plasmid

    #163697

    Purpose

    Expresses c-terminal half of TurboID on surface of astrocytes

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV-U6+27-Tornado-Broccoli

    Plasmid

    #124360

    Purpose

    Overexpression of circular RNA (Broccoli)

    Depositor

    Samie Jaffrey

    Article

    Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

    Insert

    Tornado-Broccoli
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSIQmate-EGFP

    Plasmid

    #216241

    Purpose

    All-in One IQ-Cumate transgene switch for the EGFP expression

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MCP-M-MLV RT∆RNase H

    Plasmid

    #213749

    Purpose

    For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2881

    Plasmid

    #144254

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMPRAdonor2

    Plasmid

    #49353

    Purpose

    minP-luc2 donor plasmid for MPRA library construction

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    luc2
    

    Use

    Luciferase

    Promoter

    minP

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-S33Y Beta-catenin

    Plasmid

    #19286

    Purpose

    Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S33Y (presumptive GSK3β phosphorylation site)

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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