164,223 results

1. pMRX-IZ-HaloTag7-CHMP4A

   Plasmid

   #184895

   Purpose

   Stable expression of HaloTag7-CHMP4A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   CHMP4A (CHMP4A Human)
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132397

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1058A

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AARS_C2_pNIC-Bio3

   Plasmid

   #153047

   Purpose

   Expression construct for producing Avi tagged Alanyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   AARS (AARS Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF RBP-FL P53

   Plasmid

   #174042

   Purpose

   Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

   Depositor

   Stewart Loh

   Article

   Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

   Insert

   P53 gene (TP53 Human)
   

   Use

   Tags

   Thermoanaerobacter Tencongenesis ribose binding p…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMH-MBP

   Plasmid

   #101768

   Purpose

   Gateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion protein

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-6P-SEW-YC3.6

   Plasmid

   #31379

   Depositor

   Fritjof Helmchen

   Article

   Minderer et al J Physiol. 2011 Nov 14.

   Insert

   Yellow Cameleon 3.60
   

   Use

   AAV

   Tags

   Venus and eCFP

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriExRhoA2G

   Plasmid

   #40176

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG BacMam

   Plasmid

   #160451

   Purpose

   vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

   Depositor

   Eric Gouaux

   Article

   Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

   Plasmid

   #224006

   Purpose

   AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid

    #230058

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    Tags

    6X HIS, HRV 3C PreScission protease, HaloTag, and…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid

    #230059

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 single chain variable fragment (scFv)
    

    Use

    Tags

    6X HIS, HRV 3C PreScission protease, HaloTag, and…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ shcGAS-Hsa

    Plasmid

    #128175

    Purpose

    Doxycyclin inducible shRNA knockdown of human cGAS gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shcGAS Mmu (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_PA-Cre

    Plasmid

    #122960

    Purpose

    Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDH3-PA-GFP

    Plasmid

    #18819

    Depositor

    Wei-Lih Lee

    Article

    Vorvis et al Yeast. 2008 Sep;25(9):651-9.

    Insert

    Photoactivatable Green Fluorescent Protein
    

    Use

    Yeast tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. SuExp-hPLD4

    Plasmid

    #201244

    Purpose

    express full length human PLD4 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD4 (PLD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNCS BFP

    Plasmid

    #91757

    Purpose

    Bacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

    Insert

    Blue Fluorescent Protein
    

    Use

    Tags

    Hexa-Histidine, Xpress epitope for detection with…

    Expression

    Bacterial

    Mutation

    GFP (Y66H)

    Promoter

    T7

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCH1/RAD51o

    Plasmid

    #102562

    Purpose

    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

    Depositor

    Maria Spies

    Article

    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

    Inserts

    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli Expression

    Promoter

    T7

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD649-HAsp-SIGLEC8-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156792

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SIGLEC8 (SIGLEC8 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOpen-MMLV_RT (lack H)

    Plasmid

    #165556

    Purpose

    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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