164,223 results

1. GPRC5D-DuET

   Plasmid

   #213298

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPRC5D (GPRC5D Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

   Plasmid

   #115519

   Purpose

   To construct multiple sgRNA expression vector for CRISPR

   Depositor

   Xingxu Huang

   Insert

   ccdB-sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBb(RSF1010)1k-GFPuv

   Plasmid

   #106395

   Purpose

   IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJC5-3XHA-EGFP-PEX26

   Plasmid

   #139054

   Purpose

   HA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

   Depositor

   David Sabatini

   Article

   Ray et al iScience. 2020 May 22;23(5):101109. doi:

   Insert

   3XHA-EGFP-PEX26
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   UBC

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUdOm1.1

   Plasmid

   #210273

   Purpose

   Empty backbone with ampicillin resistance for high expression in mammalian cells

   Depositor

   Corrie daCosta

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCM184-ParB2::eGFP

   Plasmid

   #87252

   Purpose

   Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB2
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-ESR1-L536Q

   Plasmid

   #116369

   Purpose

   Lentiviral expression of ESR1 L536Q

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L536Q

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207180

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SMARCA2-GFP

   Plasmid

   #65390

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   SMARCA2 (SMARCA2 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. InterCatch-GFP

    Plasmid

    #186900

    Purpose

    Cell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10

    Depositor

    Allen Liu, Taekjip Ha

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    TfR-sfGFP-SpyCatcher003-sfCherry1-10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

    Plasmid

    #204821

    Purpose

    Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

    Depositor

    Emma Rawlins

    Article

    He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

    Insert

    An upstream region including human SCGB3A2 promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SCGB3A2 promoter

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. 139H2_HC

    Plasmid

    #206201

    Purpose

    For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
    

    Use

    Tags

    -AAAHHHHHHHH

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-HA-MEKK1-1-1493

    Plasmid

    #21632

    Depositor

    Melanie Cobb

    Article

    Xu et al Proc Natl Acad Sci U S A. 1995 Jul 18. 9

    Insert

    mitogen-activated protein kinase kinase kinase 1 (Map3k1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pUASTattb-cAMPr Y205A, R739A

    Plasmid

    #84933

    Purpose

    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr Y205A R739A
    

    Use

    Tags

    Expression

    Insect

    Mutation

    PKA-C Y205A, PKA-R R739A

    Promoter

    5X UAS

    Available Since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pREP7-RLuc

    Plasmid

    #124893

    Purpose

    This plasmid is used with pREP4-luc as a control.

    Depositor

    Keji Zhao

    Article

    Liu et al Cell. 2001 Aug 10;106(3):309-18.

    Insert

    RLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

17. InterTag-BFP

    Plasmid

    #186901

    Purpose

    Cell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTag

    Depositor

    Allen Liu, Taekjip Ha

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    SpyTag003-sfCherry11-tagBFP-PDGFR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

    Plasmid

    #100050

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    hChR2(E123T/H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized, E123T/H134R

    Promoter

    CaMKIIa

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3543 pMIG Bcl-XL mut8

    Plasmid

    #8792

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bcl-XL (BCL2L1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G138A, R139L, I140N

    Promoter

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

20. 137_pAAV-ProA3-CatCh-GFP-WPRE

    Plasmid

    #125887

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProA3

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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