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pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Publication

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSV Sport PPAR gamma 2

Plasmid

#8862

Depositor

Bruce Spiegelman

Publication

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

PPAR gamma (Pparg Mouse)

Expression

Mammalian

Available since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.27_ARE/NRF2-SPE

Plasmid

#177775

Purpose

Luciferase reporter for ARE/NRF2

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

NRF2 (NFE2L2 Human)

Use

Luciferase

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Publication

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA3mt

Tags

Myc

Expression

Mammalian

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GST-RH▲-MNase

Plasmid

#136292

Purpose

Expression of catalytic inactive RNase H fused to micrococcal nuclease

Depositor

Kavitha Sarma

Publication

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

RNase HI-D10R-E48R and MNase

Expression

Bacterial

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Publication

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Publication

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Tags

No tag

Expression

Mammalian

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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M23: pHR-SFFVp-FUSN::mCherry::SspB

Plasmid

#122148

Purpose

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Publication

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

mCherry-sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-214

Promoter

SFFV

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FKBP-V5-AP-nes_pLX304

Plasmid

#120912

Purpose

Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

FKBP-V5-AP-NES

Use

Lentiviral

Expression

Mammalian

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR CD19-FcGamma CAR

Plasmid

#113014

Purpose

for production of lentivirus encoding the CD19 CAR-Pfcgamma

Depositor

Ron Vale

Publication

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P intracellular GFP (CD19 Human, Synthetic, Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

Depositor

David Sabatini

Publication

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDH-puro-EWS-FLI1

Plasmid

#102813

Purpose

Overexpress EWS-FLI1. 3rd generation lentiviral vector.

Depositor

Jialiang Wang

Publication

Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

Insert

EWS-FLI1 (EWSR1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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STARR-seq luciferase validation vector_ORI_empty

Plasmid

#99297

Purpose

Luciferase validation vector; empty vector without insert to determine baseline

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Publication

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc. Inducible with tetracycline or doxycycline.

Depositor

Takuji Iwasato

Publication

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Promoter

EF-1 alpha

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTRC-APEX2

Plasmid

#72558

Purpose

Expression of cytosolic APEX2 in bacteria

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Tags

His6

Expression

Bacterial

Mutation

A134P relative to APEX

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pdCas9-DNMT3A-PuroR_v2

Plasmid

#74407

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, Synthetic, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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