Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35024||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)7000
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
Tpr MUST be grown in STBL2 cells (Gibco/Invitrogen). Refer to manufacturer protocol that comes with the cells, grow everything at 30 degrees. Tpr seems to be recombinogenic, and STBL2 cells help with that.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EGFP-Tpr was a gift from Larry Gerace (Addgene plasmid # 35024 ; http://n2t.net/addgene:35024 ; RRID:Addgene_35024)
For your References section:Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export. Frosst P, Guan T, Subauste C, Hahn K, Gerace L. J Cell Biol. 2002 Feb 18;156(4):617-30. Epub 2002 Feb 11. 10.1083/jcb.200106046 PubMed 11839768