166,201 results

1. MC3R-Tango

   Plasmid

   #66429

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   MC3R (MC3R Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. bZiPro

   Plasmid

   #86846

   Purpose

   express NS2B-NS3 protease in E.coli

   Depositor

   Dahai Luo

   Article

   Zhang et al Science. 2016 Dec 8. pii: aai9309.

   Inserts

   NS3 1-177
   NS2B45-96
   

   Tags

   a hexahistidine tag and a thrombin cleavage site

   Expression

   Bacterial

   Mutation

   NS2B cofactor region (residues 45–96) and NS3 pro…

   Promoter

   T7

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMarC9-R6k

   Plasmid

   #89477

   Purpose

   transposon plasmid, compatible with Tn-Seq

   Depositor

   George Church

   Article

   Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

   Insert

   pMarC9-R6k
   

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFX-Tom20-EGFP

   Plasmid

   #174186

   Purpose

   Express EGFP with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with mitchondria-targeting sequence
   

   Tags

   the mitochondria-targeting sequence of TOM20

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA Myc DBC1

   Plasmid

   #35096

   Depositor

   Osamu Hiraike

   Article

   Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

   Insert

   deleted in breast cancer 1 (CCAR2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcGP67A-PSMA

   Plasmid

   #11524

   Depositor

   Pamela Bjorkman

   Article

   Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

   Insert

   prostate-specific membrane antigen (FOLH1 Human)
   

   Tags

   His

   Expression

   Insect

   Mutation

   There is a short N-terminal truncation of this pr…

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRZeroT

   Plasmid

   #120276

   Purpose

   Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

   Depositor

   Ken Motohashi

   Article

   Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

   Type

   Empty backbone

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mito-SNAP

   Plasmid

   #206480

   Purpose

   Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
   

   Tags

   SNAPf

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-LMNA-gRNA1

   Plasmid

   #122507

   Purpose

   expresses WT spCas9 and a chimeric gRNA targeting human LMNA

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   LMNA-gRNA1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB6 ZO1myc

    Plasmid

    #30317

    Depositor

    James Anderson, Alan Fanning

    Article

    Fanning et al J Biol Chem. 1998 Nov 6. 273(45):29745-5

    Insert

    Zonula Occludens-1 (TJP1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCDH-puro-CMV-GC3AI

    Plasmid

    #78910

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    GC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_ERO1L_WT

    Plasmid

    #81797

    Purpose

    Gateway Donor vector containing ERO1L , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ERO1L (ERO1A Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDP0437

    Plasmid

    #221127

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    pHluorin
    

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Expression

    Insect

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-P25C-GFP

    Plasmid

    #1343

    Depositor

    Li-Huei Tsai

    Insert

    P25 (CDK5R1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. LT3GEPIR-CDK1shRNA1

    Plasmid

    #220569

    Purpose

    To inducibly knockdown CDK1 expression

    Depositor

    Sean Lee

    Insert

    CDK1 shRNA1 (CDK1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE3G (TetOP) promoter

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRPML1-HA

    Plasmid

    #18825

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAnc80L65AAP

    Plasmid

    #92307

    Purpose

    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

    Depositor

    Luk Vandenberghe

    Article

    Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

    Insert

    Ancestral AAV Capsid Anc80L65AAP
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Rep2

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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