162,109 results

1. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. DsRed-Rab5 WT

   Plasmid

   #13050

   Depositor

   Richard Pagano

   Article

   Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

   Insert

   RAB5A (Canis familiaris) (RAB5A Canis familiaris)
   

   Use

   Tags

   DsRed2 and HA

   Expression

   Mammalian

   Mutation

   asparagine 3 changed to serine (neutral); serine …

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-CMV-Galpha-s ONE-GO

   Plasmid

   #189731

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-s ONE-GO
   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. EBFP2-H2B-6

   Plasmid

   #55243

   Purpose

   Localization: Nucleus/Histones, Excitation: 383, Emission: 448

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAG1171 FIREmate-ECFP-Giantin

   Plasmid

   #205166

   Purpose

   Expresses FIREmate-ECFP-Giantin in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   FIREmate-ECFP-Giantin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiGuide-mCherry

   Plasmid

   #217005

   Purpose

   lentiviral plasmid for gRNA cloning with selectable mCherry expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28 MBP_PrA/G-H6

   Plasmid

   #176077

   Purpose

   Express His6 tagged MBP_PrA/G in Escherichia coli

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   MBP PrA/G-H6
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLIN2-V5-APEX2

   Plasmid

   #170573

   Purpose

   A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

   Depositor

   James Olzmann

   Article

   Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

   Insert

   PLIN2 (Plin2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. gCH128 (non-targeting crRNA)

   Plasmid

   #217339

   Purpose

   Non-targeting crRNA

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   crNT-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pG1

    Plasmid

    #162602

    Purpose

    Edit Ade2 gene in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nb-gp41-GFP (MoonTag-Nb-GFP)

    Plasmid

    #128602

    Purpose

    Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and sfGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-Luc

    Plasmid

    #33154

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pET-Expotin 1

    Plasmid

    #42047

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Exportin-1 (XPO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    dSV40 Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. GPR39-Tango

    Plasmid

    #66357

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR39 (GPR39 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCh-Rab5

    Plasmid

    #49201

    Purpose

    expression of Rab5 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    Rab5 (RAB5B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX ALIX-GFP WT

    Plasmid

    #138582

    Purpose

    Expresses fluorescent ALIX in mammalian cells

    Depositor

    Wesley Sundquist

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Lentiviral

    Tags

    GFP and h30

    Expression

    Mammalian

    Mutation

    Promoter

    TREtight

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. YFVdelCME-mVenus

    Plasmid

    #179953

    Purpose

    pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking C, prM, E proteins but with mVenus
    

    Use

    Transneuronal tracing

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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