166,201 results

1. FLAG-hPAX7 (#348)

   Plasmid

   #78337

   Purpose

   Overexpression of human PAX7 ORF with a N-terminal FLAG tag

   Depositor

   Matthew Alexander, Louis Kunkel

   Insert

   PAX7 (PAX7 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pET-6xHis-GFP(+30)

   Plasmid

   #199164

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+30)
   

   Tags

   MGHHHHHHGGA

   Mutation

   E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23_Mmp11

   Plasmid

   #209520

   Purpose

   Expresses luciferase under control of Mmp11 promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

   Insert

   Mmp11 promoter (Mmp11 Mouse)
   

   Use

   Luciferase

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-TBK1-C-FLAG-HA

   Plasmid

   #131792

   Purpose

   Lentiviral vector expressing WT TBK1 containing STOP codon

   Depositor

   Alicia Pickrell

   Article

   Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

   Insert

   TBK1 WT (TBK1 Human)
   

   Use

   Lentiviral

   Tags

   STOP codon

   Expression

   Mammalian

   Mutation

   WT full length

   Promoter

   CMV

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRha-ABE8e-SpCas9-NG

   Plasmid

   #201190

   Purpose

   Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

   Depositor

   Jonathan Yen

   Article

   Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

   Insert

   ABE8e-SpCas9-NG
   

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Promoter

   pRhaBAD

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL_PylRS(WT)-tRNA

   Plasmid

   #223512

   Purpose

   PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   araBAD, rrnB, and glnS and proK

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. SL-SunTag

   Plasmid

   #196919

   Purpose

   The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. V5-LOV-Turbo-NES

   Plasmid

   #199545

   Purpose

   expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NES and V5

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC39A10

    Plasmid

    #132275

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A10 (SLC39A10 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pOSIP-KH (KanR, HK022)

    Plasmid

    #45983

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. STR-KDEL_PGRN-SBP-mCherry

    Plasmid

    #180439

    Purpose

    Co-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherry

    Depositor

    Shawn Ferguson

    Article

    Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

    Insert

    Progranulin (GRN Human)
    

    Tags

    SBP-mCherry

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EMTB-mBaoJin

    Plasmid

    #229574

    Purpose

    Expresses EMTB-mBaoJin fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Expression

    Mammalian

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PuroR-2A-MLH1-SB

    Plasmid

    #228871

    Purpose

    Plasmid for MLH1-SB overexpression

    Depositor

    Sangsu Bae

    Article

    Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

    Insert

    PuroR-2A-MLH1-SB
    

    Use

    CRISPR

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG roGFP2-mito

    Plasmid

    #168509

    Purpose

    Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

    Insert

    mito-roGFP2
    

    Tags

    roGFP2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ER-mCherry-eMagA

    Plasmid

    #162248

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    CytP450 ER targeting sequencel-mCherry-eMagA
    

    Expression

    Mammalian

    Promoter

    beta-actin promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSI-Target-AID-NG

    Plasmid

    #119861

    Purpose

    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-mir30

    Plasmid

    #14758

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    mir30 cassette
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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