164,223 results

1. pCM189-ParB1::mCherry

   Plasmid

   #87253

   Purpose

   Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB1
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Cerulean-Epac2-Venus

   Plasmid

   #138196

   Purpose

   FRET-based biosensor for monitoring Epac2 activation.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-N-HA-Pcdh15_mini_V4

   Plasmid

   #199185

   Purpose

   An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

   Depositor

   David Corey

   Article

   Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

   Insert

   HA-mmPcdh15_mini_V4-CD2
   

   Use

   AAV

   Tags

   Human influenza hemagglutinin (HA)

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-Cas9-H2B-mCherry

   Plasmid

   #159655

   Purpose

   Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-neo largeTcDNA

   Plasmid

   #1780

   Purpose

   Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lamp1-mScarlet-I

   Plasmid

   #98827

   Purpose

   In vivo visualization of lysosomes (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   Lamp1
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTK91_GFP-MRLC2

   Plasmid

   #46355

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   MRLC2 (MYL12B Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   pBABE

   Available Since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-BrainbowTEC

   Plasmid

   #82405

   Purpose

   Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

   Depositor

   Judith Eisen

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Inserts

   tdTomatoMYC fusion
   EGFP
   E2CrimsonHA
   

   Use

   Tags

   HA and MYC

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-UBC-hCD24-T2A-GFP

   Plasmid

   #205456

   Purpose

   lentiviral plasmid for expression of human CD24

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD24 (CD24 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIE1

    Plasmid

    #136355

    Purpose

    Selection by Inducible Effectors

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    P1TDP-GHO23

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Villinpromoter-blue-FlpOERT2

    Plasmid

    #67278

    Purpose

    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Insert

    Villin-mTQ2-P2A-FlpO-ERT2
    

    Use

    Tags

    mTQ2, linked to FlpO through an P2A ribosomal ski…

    Expression

    Mammalian

    Mutation

    Promoter

    Villin

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTCF156 HLA-A*02:01

    Plasmid

    #180456

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*02:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-PGK-RAP1A-WT-puro

    Plasmid

    #156166

    Purpose

    Lentiviral vector for expression of RAP1A-WT, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX_314-HRI-V5

    Plasmid

    #202434

    Purpose

    Expression of HRI

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHM066

    Plasmid

    #225524

    Purpose

    Encodes Wnt-responsive destablized iRFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Insert

    iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIBR004

    Plasmid

    #177667

    Purpose

    SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ1651-sgTelomere(F+E)

    Plasmid

    #51024

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    Optimized sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pE6n

    Plasmid

    #17460

    Purpose

    modified pENTR vector for N-terminal EYFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    EYFP

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. mATF4

    Plasmid

    #24874

    Depositor

    Fumihiko Urano

    Article

    Ghosh et al PLoS One. 2010 . 5(3):e9575.

    Insert

    mouse ATF4 (Atf4 Mouse)
    

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2010

    Availability

    Academic Institutions and Nonprofits only