159,333 results
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Plasmid#200729PurposeLentiviral expression of Flag-Tagged human HNRNPQDepositorInsertHNRNPQ (SYNCRIP Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV FLEX Synaptophysin GFP
Plasmid#137188PurposeCre-dependent expression of synaptophysin-GFPDepositorInsertSynaptophysin GFP (Syp Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSG288
Plasmid#115607Purposesurface-displayed Ag3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP12042
Plasmid#222354PurposeTn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
UseTagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNos-PE2-attB
Plasmid#149549PurposeExpression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.DepositorInsertPE2
UseCRISPRTagsExpressionInsectMutationPromoternanos (nos)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR
Plasmid#182251PurposeHuman PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR3
Plasmid#183291PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR3 geneDepositorInsertFGFR3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a
Plasmid#40047DepositorInserthSlp2-a (SYTL2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_Cas9-blast_GFP
Plasmid#162074Purpose3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistanceDepositorInsertsCas9-2a-blasticidin S deaminase
EGFP
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEF1a and sv40Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSII422
Plasmid#35462DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1G511A-CMyc
Plasmid#50734PurposeProduces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 G511A (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Gly 511 to AlaPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCX137
Plasmid#229212PurposeCRISPR array plasmid for NOTCH2 (Spacers 1-24)DepositorInsertCRISPR array plasmid for NOTCH2 (Spacers 1-24)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA4 (MSW#517)
Plasmid#208070PurposeExpression of GFP-tagged hsRPA4 in human cellsDepositorInsertRPA4 (RPA4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianMutationPromotertet-onAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS_366
Plasmid#182131Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutationDepositorInsertsretron RNA
ec.86 RT
CspRecT
UseTagsExpressionMutationincorporated rifampicin-resistance-conferring don…PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL711
Plasmid#140381PurposeExpresses OtrR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-OtrR-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 F-tractin-EGFP
Plasmid#58473PurposeExpresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoterDepositorInsertF-tractin (Itpka Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIC291
Plasmid#51403PurposeRetroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)DepositorInsertSka1 IMAGE 3909951 (SKA1 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only