166,201 results

1. TFORF1496

   Plasmid

   #142805

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HHEX (HHEX Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBa.GFP-KIF16B tail 458-1317

   Plasmid

   #134624

   Purpose

   Mammalian expression of human KIF16B 458-1317

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF16B (KIF16B Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 457 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-CAG-Venus-Akaluc

   Plasmid

   #209768

   Purpose

   Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).

   Depositor

   Yohei Hayashi

   Insert

   Venus-Akaluc
   

   Use

   Luciferase; Piggybac transposon

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB90 - pAGM4723_VirGN54D

   Plasmid

   #123187

   Purpose

   Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Type

   Empty backbone

   Use

   Level 2 acceptor vector for expression in plants

   Expression

   Plant

   Mutation

   VirGN54D for increase virulence

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-GRAB_CRF1.0

   Plasmid

   #208661

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PLJR965

   Plasmid

   #115163

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR (deleted TetON) MtbCO
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP573

   Plasmid

   #98796

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-ENLYFQS-TEV WT
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2209

   Plasmid

   #142175

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB3L3 (CREB3L3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LbCas12a-crRNA

    Plasmid

    #213753

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a-crRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBBi-Pur GFP-TM

    Plasmid

    #139185

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.

    Depositor

    Neal Devaraj

    Insert

    GFP-TM
    

    Use

    Transposon

    Expression

    Mammalian

    Mutation

    fused to membrane anchor

    Promoter

    EF1α

    Available Since

    March 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-6xHis-GFP(-30)

    Plasmid

    #199167

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(-30)
    

    Tags

    MGHHHHHHGGA

    Mutation

    T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTNS3

    Plasmid

    #63127

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    P1 and Ptac

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3 Flag Erg

    Plasmid

    #66977

    Purpose

    Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (Erg Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035)

    Plasmid

    #214853

    Purpose

    Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs)

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    CoChR-EGFP
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF3427

    Plasmid

    #144903

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIB (NFIB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC18T-mini-Tn7T-hph-Ptet2

    Plasmid

    #218368

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLX1.8MitoKaede

    Plasmid

    #28133

    Depositor

    Geoffrey Owens

    Insert

    MitoKaede
    

    Use

    Lentiviral

    Tags

    COX VIII signal sequence

    Expression

    Mammalian

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221_SLC30A5

    Plasmid

    #132077

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC30A5 (SLC30A5 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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