177,821 results
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Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsSNAP-tag (SNAPf)ExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor
Plasmid#92142PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEFIRES-P-ACSL3-mCherry
Plasmid#87158PurposeFluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACSL3 (ACSL3 Human)
TagsmCherryExpressionMammalianPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-PRB
Plasmid#89130Purposemammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman progesterone receptor-B (PGR Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME-ERT2-Cre-ERT2
Plasmid#82587PurposeMiddle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombinationDepositorInsertERT2-CRE-ERT2
PromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Stargazin-GFP-LOVpep
Plasmid#80406PurposeMembrane localized GFP-tagged LOVpep. Expresses in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStargazin-GFP-LOVpep (CACNG2 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1-NuMA
Plasmid#81029PurposeExpresses human NuMA with N-terminal EGFP tag in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Flag-IDH1
Plasmid#62906PurposeExpresses wildtype IDH1 in mammalian cellsDepositorAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-MTOR-S2215Y
Plasmid#69013Purposeactivating MTOR mutationDepositorAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD286 (TagRFP^SEC^3xMyc)
Plasmid#70684PurposeTagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized TagRFP-TExpressionWormAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTQL1211YN
Plasmid#61704PurposeGateway compatible BiFC vector (pEarleyGate201-YN)DepositorTypeEmpty backboneUseBifc assayTagsYFP-N and HAExpressionPlantPromoter35SAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNase HI-D10R-E48R
TagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1219
Plasmid#61250PurposeCRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegansDepositorInsertsgRNA(F+E)
UseCRISPRExpressionWormPromoterU6 promoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato
Plasmid#60661PurposeFlp-dependent expression of Kir2.1-T2A-tdTomatoDepositorAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFCcGi
Plasmid#59324PurposeInducible expression of GFP and constitutive expression of mCherryDepositorInsertsmCherry
EGFP
ExpressionBacterialPromoterPbad and rpsMAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV HA hRB-wt
Plasmid#58905Purposemammalian expression of wild type human RbDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRb (RB1 Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
UseLentiviralExpressionMammalianPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NPC1 His6 EGFP
Plasmid#53521DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNPC1
TagsEGFP and His6ExpressionMammalianPromoterCMVAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by