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pcDNA3.1(+)_BetaActin_ALFA_229230

Plasmid

#250439

Purpose

Expresses tagged beta cytoplasmic actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T229/A230.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Beta Cytoplasmic Actin (ACTB Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pDisplay-hpGRISZ

Plasmid

#248027

Purpose

Plasmid for mammalian expression of hpGRISZ (synaptic zinc sensor)

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2026 Mar 3. doi: 10.1021/acsse

Insert

hpGRISZ

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE58

Plasmid

#247035

Purpose

IRF1 pathway reporter; lentiviral expression of IRF1 DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF1

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201_PARP1

Plasmid

#240317

Purpose

Entry vector for Gateway with PARP1

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARP1 (PARP1 Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBL071

Plasmid

#239089

Purpose

Expresses cCadC-AB42 under the control of Ptet

Depositor

Tina Wang

Article

Lee et al Nat Chem Biol. 2025 Jul 15. doi: 10.1038

Insert

cCadC-AB42

Use

Synthetic Biology

Tags

cCadC fused to AB42

Promoter

PTet

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-PuroR

Plasmid

#242415

Purpose

Spectral unmixing: mScarlet3 only

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-PuroR

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mStayGold-WPRE (JDW 1384)

Plasmid

#242550

Purpose

Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mStayGold (monomeric StayGold)

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mLRRC45 (158 a.a.)

Plasmid

#240067

Purpose

ITT reporter plasmid for cold and osmotic stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

LRRC45 C-terminal 158 a.a. (Lrrc45 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-TRE>hHES3:T2A:EGFP

Plasmid

#236630

Purpose

Hygromycin selected lentiviral construct with tetracyclin-inducible human HES3

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

HES3 (HES3 Human)
EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized based on a variant of wildtype GF…

Promoter

TRE

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mutCKAR3

Plasmid

#238412

Purpose

Mammalian expression of a phosphorylation deficient (T to A) mutant of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

phosphorylation deficient mutant of CKAR3 (T to A)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-10

Plasmid

#228969

Purpose

For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP6

Plasmid

#218245

Purpose

1) Ectopic expression of MAAP6 protein in mammalian cells. 2) Packaging MAAP6 sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein from Adeno-Associated Virus 6 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP9

Plasmid

#218246

Purpose

1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-Aurora B KD

Plasmid

#184043

Purpose

Expresses H2B-GFP fused to Aurora B kinase dead

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Tags

EGFP and H2B

Expression

Mammalian

Mutation

Changed Lysine 65 to Arginine

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2591

Plasmid

#144211

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF354C (ZNF354C Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BALF0/1

Plasmid

#198588

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BALF0/1 (BALF1 Epstein-Barr virus)

Use

Gateway Cloning

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBLF2/3

Plasmid

#195831

Purpose

Gateway Entry Clone of Epstein-Barr virus BBLF2/3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBLF2/3 (BBLF2/BBLF3 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcRF1(vTBP)

Plasmid

#195832

Purpose

Gateway Entry Clone of Epstein-Barr virus BcRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcRF1 (BcRF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBLF4

Plasmid

#195833

Purpose

Gateway Entry Clone of Epstein-Barr virus BBLF4

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBLF4 (BBLF4 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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