164,247 results

1. pRK5-Myc-Parkin

   Plasmid

   #17612

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOSIP-KO (KanR, 186)

   Plasmid

   #45985

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET16B.Pfu

   Plasmid

   #12509

   Purpose

   Bacterial expression of Pfu DNA polymerase

   Depositor

   Sung-Hou Kim

   Article

   Goldman et al Acta Crystallogr D Biol Crystallogr. 199

   Insert

   Pfu DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y218H, Q462R, F533L, K559I and V683A (mutations w…

   Promoter

   Available Since

   Sept. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. FL-BCAN-lentiviral-GFP

   Plasmid

   #29704

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   Brevican (BCAN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pACYC-Skp-GroEL/ES

   Plasmid

   #83922

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Gro EL/ES
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-krt4-GFP

   Plasmid

   #97151

   Purpose

   label apical epithelial cells in zebrafish

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   AcGFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-ZAP70

   Plasmid

   #23887

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ZAP70 (ZAP70 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP L138 Reporter

   Plasmid

   #119130

   Purpose

   Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   mCherry and eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   eGFP L138S

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-flag-GFP-Ecad

    Plasmid

    #205188

    Purpose

    This vector encodes of a synCAM (Ecad) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Ecad and GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. HDAC1 Flag

    Plasmid

    #13820

    Purpose

    Mammalian expression of human histone deacetylase 1 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF RBP-FL P53

    Plasmid

    #174042

    Purpose

    Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

    Depositor

    Stewart Loh

    Article

    Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

    Insert

    P53 gene (TP53 Human)
    

    Use

    Tags

    Thermoanaerobacter Tencongenesis ribose binding p…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL50009

    Plasmid

    #50309

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    C terminal HA tag (6x Human influenza hemagglutinin)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPlatTET-gRNA2

    Plasmid

    #82559

    Purpose

    All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TLCV2-APOBEC1-YTHmut

    Plasmid

    #178950

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTHmut-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A CLIP Donor

    Plasmid

    #202689

    Purpose

    CLIP donor template to generate 2 knock-ins: GFP into ACTB, and mCherry in RAB11A.

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Inserts

    CopGFP
    mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMH-MBP

    Plasmid

    #101768

    Purpose

    Gateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion protein

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCoPURO

    Plasmid

    #17533

    Depositor

    Francis Castellino

    Article

    Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

    Insert

    Puromycine Acetyl Transferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-6P-SEW-YC3.6

    Plasmid

    #31379

    Depositor

    Fritjof Helmchen

    Article

    Minderer et al J Physiol. 2011 Nov 14.

    Insert

    Yellow Cameleon 3.60
    

    Use

    AAV

    Tags

    Venus and eCFP

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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