162,109 results

1. pS-cr:GFP

   Plasmid

   #196295

   Purpose

   35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
   

   Use

   CRISPR

   Tags

   SpeI-DR-BsaI-DR-SpeI

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

   Plasmid

   #238243

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb-2xKS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-AktAR2

   Plasmid

   #64932

   Purpose

   Enhanced FRET-based biosensor for monitoring Akt activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   AktAR2
   

   Use

   Tags

   Cerulean3 and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

   Plasmid

   #159904

   Purpose

   Mutagenesis of Kcnn3

   Depositor

   Larry Zweifel

   Insert

   Kcnn3 (Kcnn3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG1186 Lyn11-ECFP-FIREmate

   Plasmid

   #205168

   Purpose

   Expresses Lyn11-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   Lyn11-ECFP-FIREmate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-CMV-jGCaMP8f

   Plasmid

   #162373

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-ChroME2f-mRuby2-ST

   Plasmid

   #170160

   Purpose

   Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-mRuby2-ST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

   Plasmid

   #111581

   Purpose

   An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   myrTagRFP-T-P2A-post-eGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pfwB

   Plasmid

   #37329

   Depositor

   John Schiller

   Article

   Buck et al J Virol. 2005 Mar;79(5):2839-46.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcFLAG-FYN-WT

    Plasmid

    #74509

    Purpose

    Overexpression of FYN in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Chougule et al Oncotarget. 2016 Feb 2. doi: 10.18632/on

    Insert

    FYN (FYN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ10878

    Plasmid

    #183964

    Purpose

    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D156R, E292R, D235R, D350R

    Promoter

    CAG

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLminP_Luc2P_RE59

    Plasmid

    #90404

    Purpose

    STAT3 - Sis-inducible element (SIE) gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT3

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-RalGDS

    Plasmid

    #118315

    Purpose

    Expression of human RalGDS

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RalGDS (RALGDS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment of human RalGDS 203aa

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mfn2-YFP

    Plasmid

    #28010

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

    Insert

    mitofusin 2 (MFN2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    silent S508S

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmU6-gRNA

    Plasmid

    #53187

    Purpose

    Expresses the S. pyogenes sgRNA from the mouse U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

    Plasmid

    #20943

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available since

    July 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pAAV.CAG.(mito).cpSFGFP.HaloTag

    Plasmid

    #214925

    Purpose

    Expresses mitochondrially targeted non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJ241-FLEX

    Plasmid

    #18925

    Purpose

    FLEX switch empty backbone

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    FLEX switch
    

    Use

    Cre/Lox; Subcloning

    Tags

    Expression

    Mutation

    An internal Spe1 site is used for subcloning a ge…

    Promoter

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rictor_2 shRNA

    Plasmid

    #1854

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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