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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,238 results

Showing: 4281 - 4300 of 159238 results

  1. TFORF3131

    Plasmid
    #144607
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR5A2 (NR5A2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144607-plasmid-map-sequence-id-346763

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  3. FABP1A

    Plasmid
    #42344
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FABP1A (FABP1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  5. pLNCX chick src Y527F

    Plasmid
    #13660
    Depositor
    Joan Brugge
    Insert
    src Y527F (SRC Chicken)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Y527F. Constitutive active mutant.
    Promoter
    Available since
    Jan. 4, 2007
    Availability
    Academic Institutions and Nonprofits only
    13660-plasmid-map-sequence-id-155869

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  7. pLEX307-APN-blast

    Plasmid
    #158455
    Purpose
    Constitutive lentiviral expression of human APN.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    APN (ANPEP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158455-plasmid-map-sequence-id-308147

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  10. ZJOM90

    Plasmid
    #133647
    Purpose
    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-SS-mCherry-HDEL
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133647-plasmid-map-sequence-id-292102

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  16. pLSB-HYG

    Plasmid
    #166699
    Purpose
    Combined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    adh15 promoter and tRNA promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166699-plasmid-map-sequence-id-474294

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Showing: 4281 - 4300 of 159238 results