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p5E-pth2

Plasmid

#191428

Purpose

Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

Depositor

Timothy Erickson

Article

Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

Insert

pth2 regulatory region (pth2 Zebrafish)

Use

Tol2 gateway 5' entry vector

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pKSE401

Plasmid

#62202

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

35S and AtU6-26p

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pPSU10-30-50-100

Plasmid

#173838

Purpose

Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPAB

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo.ARRDC1

Plasmid

#38321

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMSOX17FNV

Plasmid

#206382

Purpose

Expresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

OCT4, KLF4, c-MYC, SOX17FNV (POU5F1, KLF4, MYC, SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pICH50866

Plasmid

#48022

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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lentiCRISPR v2 sgKEAP1-2

Plasmid

#186459

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Mutation

see comments section below

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2cpV

Plasmid

#37471

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D2cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pBABE FLAG-IKK2-EE puro

Plasmid

#216790

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

IKK-2 (IKBKB Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Promoter

LTR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCFD3-dU6:3gRNA

Plasmid

#49410

Purpose

expression of gRNA under control of the Drosophila U6:3 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLCO1A2

Plasmid

#132176

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1A2 (SLCO1A2 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-TetR-NLS-VP16

Plasmid

#103834

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc6a3

Plasmid

#159902

Purpose

Mutagenesis of Slc6a3

Depositor

Larry Zweifel

Insert

Slc6a3 (Slc6a3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pME-ReaChR-citrine

Plasmid

#169045

Purpose

Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-citrine

Use

Gateway entry cloning vector

Tags

citrine

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAKTaq

Plasmid

#25712

Purpose

Bacterial expression of Taq polymerase

Depositor

David Engelke

Article

Engelke et al Anal Biochem. 1990 Dec . 191(2):396-400.

Insert

Taq polymerase

Expression

Bacterial

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pBRIT HA/FLAG

Plasmid

#17519

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
10279-M30-663

Plasmid

#225663

Purpose

Lentivirus protein expression. Matched control construct for 8522-M31-663 (Addgene # 225661) gives basal expression from mCMVp for gating/thresholding

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

dsCherry

Use

Lentiviral

Promoter

mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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