|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35489||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDidier Trono
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsThis plasmid is very low copy and may require longer incubation times
Copy numberLow Copy
- Promoter 7xTCF/LEF
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer CGTCGCCGTCCAGCTCGACCAG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byWe inserted the 7xTCF/LEF promoter cassette from the M50 Super-TOP flash plasmid (Randall Moon) into the pRRLSIN.cPPT.PGK-GFP.WPRE vector (Didier Trono), replacing the PGK promoter.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TOP-GFP was a gift from Ramesh Shivdasani (Addgene plasmid # 35489 ; http://n2t.net/addgene:35489 ; RRID:Addgene_35489)
For your References section:Differential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling. Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA. Cancer Res. 2012 Feb 8. 10.1158/0008-5472.CAN-11-3222 PubMed 22318865