|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35491||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMark Mercola
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberLow Copy
- Promoter 7xTCF/LEF
- Cloning method Restriction Enzyme
- 5′ cloning site Hpa1 (not destroyed)
- 3′ cloning site Hpa1 (destroyed during cloning)
- 5′ sequencing primer CGTCGCCGTCCAGCTCGACCAG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byWe inserted a 7xTCF/LEF optimal promoter cassette into the Hpa1 site of the PGK-H2BmCherry vector.
Terms and Licenses
Articles Citing this Plasmid
Please note that the gaps between the Addgene sequencing results and the depositor's full sequence do not alter the plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TOP-GFP.mC was a gift from Ramesh Shivdasani (Addgene plasmid # 35491 ; http://n2t.net/addgene:35491 ; RRID:Addgene_35491)
For your References section:Differential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling. Horst D, Chen J, Morikawa T, Ogino S, Kirchner T, Shivdasani RA. Cancer Res. 2012 Feb 8. 10.1158/0008-5472.CAN-11-3222 PubMed 22318865