|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31122||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2894
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameubiquitin-like protein-specific protease 1
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)660
Entrez GeneULP1 (a.k.a. YPL020C, NIB1)
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Mossessova E, Lima CD. Mol Cell. 2000 May;5(5):865-76.
Note: pFGET19_Ulp1 (#64697) is a new version of pHYRS52(#31122) with kanamycin resistance.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHYRS52 was a gift from Hideo Iwai (Addgene plasmid # 31122 ; http://n2t.net/addgene:31122 ; RRID:Addgene_31122)
For your References section:Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Muona M, Aranko AS, Iwai H. Chembiochem. 2008 Dec 15. 9(18):2958-61. 10.1002/cbic.200800604 PubMed 19031436