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pHYRS52
(Plasmid #31122)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31122 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRsetA
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 2894
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ulp1
  • Alt name
    ubiquitin-like protein-specific protease 1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    660
  • Mutation
    Ulp1(403-621)
  • Entrez Gene
    ULP1 (a.k.a. YPL020C, NIB1)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Mossessova E, Lima CD. Mol Cell. 2000 May;5(5):865-76.

Note: pFGET19_Ulp1 (#64697) is a new version of pHYRS52(#31122) with kanamycin resistance.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYRS52 was a gift from Hideo Iwai (Addgene plasmid # 31122 ; http://n2t.net/addgene:31122 ; RRID:Addgene_31122)
  • For your References section:

    Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Muona M, Aranko AS, Iwai H. Chembiochem. 2008 Dec 15. 9(18):2958-61. 10.1002/cbic.200800604 PubMed 19031436