|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35612||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3000
Modifications to backboneTAT domain (YGRKKRRQRRR) with 5' 6xHIS and 3' HA followed by MCS consisting of NcoI-KpnI-AgeI-XhoI-SphI-EcoRI-BstBI. See attached map for full cloning information.
Vector typeBacterial Expression
- Promoter T7
/ Fusion Proteins
- 6xHIS (N terminal on backbone)
- TAT (N terminal on backbone)
- HA (N terminal on backbone)
Growth in Bacteria
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTAT-HA was a gift from Steven Dowdy (Addgene plasmid # 35612 ; http://n2t.net/addgene:35612 ; RRID:Addgene_35612)
For your References section:Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration. Nagahara H, Vocero-Akbani AM, Snyder EL, Ho A, Latham DG, Lissy NA, Becker-Hapak M, Ezhevsky SA, Dowdy SF. Nat Med. 1998 Dec;4(12):1449-52. 10.1038/4042 PubMed 9846587