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plko.1/shMCT4 477

Plasmid

#99598

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-sRGECO

Plasmid

#137128

Purpose

Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E217D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJRA-GW

Plasmid

#209333

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMCSG7(gfcC)

Plasmid

#176197

Purpose

gfcC protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcC

Tags

6xHis-TeV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-puro

Plasmid

#208031

Purpose

Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

Plasmid

#121824

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/LSD1 (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_p.P29S

Plasmid

#81300

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Mutation

P29S, T135I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Flex-mGFP-APEX2

Plasmid

#171936

Purpose

Reporter for correlated light and electron microscopy.

Depositor

David Berson

Article

Stedehouder et al Elife. 2019 Nov 19;8. pii: 48615. doi: 1

Insert

mGFP-APEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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IDG_GPR89B_OE_1

Plasmid

#161700

Purpose

Overexpresses GPR89B

Depositor

Mike McManus

Insert

GPR89B (GPR89B Human)

Tags

V5

Expression

Mammalian

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEF‐PIK3IP1

Plasmid

#49214

Purpose

expresses PIK3IP1 in mammalian cells

Depositor

Lawrence Kane

Article

DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

Insert

PIK3IP1 (Pik3ip1 Mouse)

Tags

6X-his and myc

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
LbCas12a-niCPE

Plasmid

#213747

Purpose

For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-nLbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A in LbCas12a

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pTFAP2B.1.0-gDNA

Plasmid

#113791

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFAP2B

Depositor

Kevin White

Insert

TFAP2B (TFAP2B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTFAP2B-donor

Plasmid

#113823

Purpose

CRISPR donor plasmid to tag human transcription factor TFAP2B with GFP

Depositor

Kevin White

Insert

TFAP2B homology arms flanking EGFP-IRES-Neo cassette (TFAP2B Human)

Use

CRISPR

Mutation

Unknown

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP Δvpu/env

Plasmid

#101345

Purpose

Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta vpu/Env

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-PT7-RiboJ-sfGFP-MGapt

Plasmid

#129119

Purpose

Expresses a fluorescent reporter for the quantification of cell-free protein expression.

Depositor

Matthew Lux

Article

Cole et al ACS Synth Biol. 2019 Sep 20;8(9):2080-20

Insert

Superfolder GFP with Malachite Green aptamer

Use

Synthetic Biology

Tags

His6 and RiboJ Insulator

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-THP-Synaptophysin-pHluorin

Plasmid

#242903

Purpose

Fluorescent reporter for imaging of synaptic vesicle recycling in Dopaminergic Neurons

Depositor

Timothy Ryan

Article

Pulido et al Proc Natl Acad Sci U S A. 2025 Dec 16;12

Insert

synaptophysin plus pHluorin

Use

AAV

Tags

mouse synaptophysin

Expression

Mammalian

Promoter

rat Tyrosine hydroxylase

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Puro_GFP-longENSA

Plasmid

#248797

Purpose

Overexpression of long ENSA

Depositor

Maayan Salton

Article

Baker et al RNA. 2025 Dec 24:rna.080661.125. doi: 10

Insert

ENSA long isoform (ENSA Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Puro_GFP-shortENSA

Plasmid

#248796

Purpose

Overexpression of short ENSA

Depositor

Maayan Salton

Article

Baker et al RNA. 2025 Dec 24:rna.080661.125. doi: 10

Insert

ENSA short isoform (ENSA Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-Puro-Arpp19-short

Plasmid

#248798

Purpose

Overexpression of ARPP19 short

Depositor

Maayan Salton

Article

Baker et al RNA. 2025 Dec 24:rna.080661.125. doi: 10

Insert

ARPP19 short isoform (ARPP19 Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Dec. 8, 2025

Availability

Academic Institutions and Nonprofits only

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