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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.PHF19

Plasmid

#125168

Purpose

Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF19 (PHF19 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mCherry FP

Plasmid

#52114

Purpose

a bicistronic IRES-mCherry containing retroviral vector

Depositor

Dario Vignali

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 BRGI-Flag

Plasmid

#19143

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pC0096 RESCUE truncation (Cas13 delta 892-1095)

Plasmid

#130663

Purpose

CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b(delta 892-1095)-ADAR2dd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.PHF1

Plasmid

#125167

Purpose

Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

PHF1 (PHF1 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pReg-RamR

Plasmid

#190610

Purpose

Expresses the RamR transcription factor in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

RamR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

Plasmid

#164457

Purpose

AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minCMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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SlugCas9

Plasmid

#163793

Purpose

Expresses SlugCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBEVY-GU

Plasmid

#51229

Purpose

bi-directional expression vector for yeast, galactose inducible, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1,10

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-3xFLAG

Plasmid

#207684

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-IRES-Gβγ-BERKY3

Plasmid

#158426

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGWB415

Plasmid

#74809

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurboID

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Str-Ii_SBP-mCherry-Golgin84

Plasmid

#65304

Purpose

synchronize trafficking of Golgin-84 from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLT61

Plasmid

#1690

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T7p, unc224616, unc2218148, unc2218082, unc223827…

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pQCXIP-VEGFC

Plasmid

#73012

Purpose

Retroviral expression of human VEGFC

Depositor

Michael Grusch

Insert

VEGFC (VEGFC Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_WT

Plasmid

#81760

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Jesse Boehm, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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