161,256 results

1. pLX304 CMV HA-HaloTag-FRB-sTurboID (C)

   Plasmid

   #153003

   Purpose

   Expresses low-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CVB3 IRES

   Plasmid

   #189968

   Purpose

   Part 2 plasmid to introduce the CVB3 IRES into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   CVB3 IRES
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFH2.22_mCherry-MS2x8

   Plasmid

   #205537

   Purpose

   Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-UCOE-SFFV-Zim3-dCas9-BFP

   Plasmid

   #188776

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-link-ytdTomato-URA3

   Plasmid

   #168054

   Purpose

   Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Use

   Tags

   ytdTomato

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV4-3 HA/IkB-alpha

   Plasmid

   #21985

   Depositor

   Warner Greene

   Article

   Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

   Insert

   I-kappa B alpha (NFKBIA Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX_HA

   Plasmid

   #178534

   Purpose

   Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-PV-NLS-GFP

   Plasmid

   #17300

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-GFP-NLS (Pvalb Rat)
   

   Use

   Tags

   Myc and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Use

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mKeima-Red-N1

    Plasmid

    #54597

    Purpose

    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mKeima-Red

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

    Plasmid

    #116931

    Purpose

    Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    lumazine synthase
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NL4-3 mCherry Luciferase

    Plasmid

    #44965

    Purpose

    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

    Depositor

    Warner Greene

    Article

    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371

    Insert

    mCherry, Luciferase, IRES
    

    Use

    Lentiviral

    Tags

    Luciferase, IRES and mCherry with D212G

    Expression

    Mutation

    S34C mutation in Nef

    Promoter

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5-HA-YFP-rictor

    Plasmid

    #73387

    Purpose

    Expresses HA and YFP tagged Rictor

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

    Plasmid

    #160141

    Purpose

    Cre- dependent expression of Sun1GFP for nuclei isolation

    Depositor

    Andreas Pfenning

    Article

    Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

    Insert

    Sun1-GFP (Sun1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-truncated: removed amino acids 1-207, retained …

    Promoter

    Ef1a

    Available since

    March 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-HA-NONO

    Plasmid

    #127655

    Purpose

    Over-express HA-tagged human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ADRA2B-Tango

    Plasmid

    #66217

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADRA2B (ADRA2B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-EGFP-2xFYVE

    Plasmid

    #136996

    Purpose

    Lentiviral EGFP lipid sensor for PI(3)P

    Depositor

    Ken-Ichi Takemaru

    Insert

    EGFP-2xFYVE
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pINDUCER20-α-Syn

    Plasmid

    #92200

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for α-Syn

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    SNCA (SNCA Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge