165,343 results

1. pLKO.1 mCherry

   Plasmid

   #128073

   Purpose

   puro exchanged to mCherry

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   no

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx4-H6-FGAm (FlincG3)

   Plasmid

   #49202

   Purpose

   Fluorescent reporter for cGMP

   Depositor

   John Garthwaite

   Article

   Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

   Insert

   FlincG3
   

   Tags

   His tag + other tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Met to Lys in cpEGFP region similar to CGaMP3

   Promoter

   CMV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2-RB1

   Plasmid

   #87836

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Insert

   sgRB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Expression

   Mammalian

   Promoter

   Tight TRE promoter and U6

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-ihSyn1-tTA

   Plasmid

   #99120

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-AKAR4-Kras

   Plasmid

   #61621

   Purpose

   Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4-Kras
   

   Tags

   C-terminal targeting sequence from Kras, Cerulean…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdTomato-H2B-6

   Plasmid

   #58101

   Purpose

   Localization: Nucleus/Histones, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   H2B (H2BC11 Human)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-BRCA1

   Plasmid

   #115491

   Purpose

   EGFP-BRCA1 expression vector. Parton clone BXY

   Depositor

   Rob Parton

   Article

   McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1009R (See depositor comments)

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

   Plasmid

   #218527

   Purpose

   sgRNA targeting human EIF2AK2/PKR

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-Puro_hCOL4A2-Myc-His-WPRE

   Plasmid

   #198080

   Purpose

   Express hCOL4A2 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A2 (COL4A2 Human)
   

   Tags

   TEV-Myc-His

   Expression

   Mammalian

   Promoter

   CMV, beta-globin/IgG chimeric intron

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. ALPaGA

    Plasmid

    #175272

    Purpose

    Lactate biosensor in anaerobic conditions

    Depositor

    Jerome Bonnet

    Article

    Zuniga et al ACS Synth Biol. 2021 Dec 1. doi: 10.1021

    Insert

    LldR_Synthetic_promoter_sfGFP
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTP264

    Plasmid

    #149334

    Purpose

    Bacterial expression of Biotin ligase BirA

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Biotin ligase BirA
    

    Tags

    His14 and bdNEDD8

    Expression

    Bacterial

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_HEY1_NCOA2

    Plasmid

    #205829

    Purpose

    Express mEGFP-tagged fusion protein, HEY1_NCOA2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    HEY1_NCOA2 (HEY1, NCOA2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ApoE4

    Plasmid

    #197148

    Purpose

    Expresses ApoE4 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE4 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid

    #239102

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Donor plasmid for integration into the human geno…

    Tags

    p2A-tdTomato-p2A-Thy1.2

    Expression

    Mammalian

    Promoter

    Endogenous POU4F2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. E1b-GFP-Tol2

    Plasmid

    #37845

    Depositor

    Nadav Ahituv

    Article

    Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

    Type

    Empty backbone

    Use

    Zebrafish enhancer assay vector

    Promoter

    E1b

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. AKT-translocation-mStrawberry reporter

    Plasmid

    #158685

    Purpose

    This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCgsgRNA_crtYf

    Plasmid

    #153338

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    crRNA targeting crtYf
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRZeroT

    Plasmid

    #120276

    Purpose

    Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-SFPQ

    Plasmid

    #166960

    Purpose

    Expresses FLAG-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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