162,999 results

1. tau/pET29b

   Plasmid

   #16316

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   tau (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5 BRGI-Flag

   Plasmid

   #19143

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. spCas9-BlastR (pCBhCas9-BlastR)

   Plasmid

   #71489

   Purpose

   Expresses SpCas9 and Blasticidin resistance

   Depositor

   Richard Sherwood

   Article

   Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

   Insert

   spCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CBh

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

   Plasmid

   #154773

   Purpose

   plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y125A in G1PylS, hybrid PylT with A41AA and C55A …

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-endo-GsCT

   Plasmid

   #205516

   Purpose

   Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-GsCT (GNAS Human, Synthetic)
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDY1173_ADAR1p150

   Plasmid

   #193192

   Purpose

   Express full length ADAR1p150

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   ADAR1p150 (ADAR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFV2-HA

   Plasmid

   #187082

   Purpose

   Expression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)

   Depositor

   Steven Vik

   Article

   Bae et al Biochim Biophys Acta. 2009 Sep;1787(9):1

   Insert

   atpBEFHAGDC
   

   Use

   Tags

   6-His beta subunit (atpD) and HA tag subunit a (a…

   Expression

   Bacterial

   Mutation

   All Cys codons changed to Ala.

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9-sgRNA-RFP

   Plasmid

   #166005

   Purpose

   Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

   Depositor

   Martin Buck

   Article

   Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FASTHDR-Cterm-HaloTag-Puromycin

   Plasmid

   #167208

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEJS2424_Nme2.Smu-ABE8e-i8

    Plasmid

    #201522

    Purpose

    Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i8

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-i8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRISPR-StAR4BN (Lenti)

    Plasmid

    #222692

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. T7 promoter + terminators reporter plasmid

    Plasmid

    #156456

    Purpose

    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    NeoR/KanR
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7 and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-DJ-N589X

    Plasmid

    #130878

    Purpose

    Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

    Depositor

    Prashant Mali

    Article

    Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

    Insert

    AAV-DJ cap N589X
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    N589X

    Promoter

    Unknown

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-IQGAP1

    Plasmid

    #30112

    Depositor

    David Sacks

    Article

    Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    tandem EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EndophilinA1-mRuby2

    Plasmid

    #171945

    Purpose

    Expresses Endophilin A1 in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Endophilin A1 (SH3GL2 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS418

    Plasmid

    #11256

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAK1

    Plasmid

    #73244

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AAK1 (AAK1 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 47-307

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only