170,715 results

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GPX4

Plasmid

#38797

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GPX4 (GPX4 Human)

Tags

His Tev

Expression

Bacterial

Mutation

SeCys73 -> Gly

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP1-10

Plasmid

#68715

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

GFP1-10

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR1

Plasmid

#116740

Purpose

Lentiviral expression of FGFR1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR1 (FGFR1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2-BSD

Plasmid

#161514

Purpose

Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

Insert

PE2-P2A-BSD

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CBFA2T3_GLIS2

Plasmid

#205786

Purpose

Express mEGFP-tagged fusion protein, CBFA2T3_GLIS2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CBFA2T3_GLIS2 (CBFA2T3, GLIS2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-eCas9-GFP-U6-gRNA

Plasmid

#79145

Purpose

All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

Depositor

Jizhong Zou

Insert

eSpCas9(1.1)

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTW335h3

Plasmid

#237326

Purpose

expresses O. sativa RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Inserts

O. sativa RbcL
O. sativa RbcS-6xHis

Tags

6xHis and None

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pICH47742

Plasmid

#48001

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry2-C1

Plasmid

#54563

Purpose

Localization: C1 Cloning Vector, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

mCherry2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pFlp-Tc

Plasmid

#222336

Purpose

Expresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sacB (sacB )
flp (flp )
cI (cI )

Promoter

cl, flp, and sacB

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen

Plasmid

#189976

Purpose

Part 3-4-5 plasmid to introduce mNeonGreen into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF2959

Plasmid

#144435

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KAT2A (KAT2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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c-myc-PT3EF1a

Plasmid

#92046

Purpose

Expresses c-Myc in mammalian cells

Depositor

Xin Chen

Article

Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

Insert

C-MYC (MYC Human)

Expression

Mammalian

Promoter

EF-1a

Available Since

July 15, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-CDT2

Plasmid

#215091

Purpose

Expresses FLAG-CDT2 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDT2 (DTL Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-VC3AI

Plasmid

#78907

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

VC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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