pBS MCK promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12528||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameMCK promoter
Alt namemuscle creatine kinase promoter
SpeciesM. musculus (mouse)
Insert Size (bp)6500
Entrez GeneCkm (a.k.a. CPK-M, Ckmm, M-CK, MCK)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
A 6.5 kb XhoI fragment containing 3 kb of the MCK promoter and enhancer 1, untranslated exon 1, 3 kb of intron 1 including the enhancer region E2, and the first 16 bp of exon 2 was subcloned into the XhoI site of pBluescriptKS to result in pBSMCK. A PolyA sequence was added downstream (see author's map, MCK-polyA-2). Please note that the restriction sites shown between the MCK promoter and polyA region in the author's map are NOT unique sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS MCK promoter was a gift from Ronald Kahn (Addgene plasmid # 12528 ; http://n2t.net/addgene:12528 ; RRID:Addgene_12528)
For your References section:A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance. Bruning JC, Michael MD, Winnay JN, Hayashi T, Horsch D, Accili D, Goodyear LJ, Kahn CR. Mol Cell. 1998 Nov . 2(5):559-69. 10.1016/S1097-2765(00)80155-0 PubMed 9844629