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pBS aP2 promoter (5.4kb) polyA
(Plasmid #11424)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11424 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript SK II +
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aP2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5400
  • GenBank ID
    M84651
  • Entrez Gene
    Fabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PolyA PCR'd from pSG5 and inserted into the NotI and SacII sites of the plasmid pBS aP2 promoter (5.4kb). The polyA sequence is approximately 250bp and encompasses an additional EcoRI site.

An EcoRI site is also present at -1.7 kb in the aP2 promoter.

Addgene has compiled full sequence for this plasmid. Please see Notes link.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS aP2 promoter (5.4kb) polyA was a gift from Ronald Kahn (Addgene plasmid # 11424 ; http://n2t.net/addgene:11424 ; RRID:Addgene_11424)