pBS aP2 promoter (5.4kb) polyA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11424||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK II +
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert nameaP2 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)5400
Entrez GeneFabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)
- Cloning method Restriction Enzyme
- 5′ cloning site See map (not destroyed)
- 3′ cloning site See map (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. Bruce Spiegelman
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
PolyA PCR'd from pSG5 and inserted into the NotI and SacII sites of the plasmid pBS aP2 promoter (5.4kb). The polyA sequence is approximately 250bp and encompasses an additional EcoRI site.
An EcoRI site is also present at -1.7 kb in the aP2 promoter.
Addgene has compiled full sequence for this plasmid. Please see Notes link.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS aP2 promoter (5.4kb) polyA was a gift from Ronald Kahn (Addgene plasmid # 11424 ; http://n2t.net/addgene:11424 ; RRID:Addgene_11424)