aP2-luciferase
(Plasmid #8858)

• Depositing Lab: Bruce Spiegelman
• Publication: Graves et al Mol Cell Biol. 1992 Mar . 12(3):1202-8.

Backbone

• Vector backbone: pGL3-promoter
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5010
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aP2 enhancer / promoter
• Alt name: aP2
• Species: M. musculus (mouse)
• Insert Size (bp): 520
• GenBank ID: M84651
• Entrez Gene: Fabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)
• Tag / Fusion Protein:
  • luciferase reporter (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid created by James Rhee by cloning 520bp of the aP2 enhancer from aP2-CAT into the pGL2 luciferase reporter. This is the abbreviated insert sequence:
ggtaccGAATTCCAGCAGGAATCA.......GGATCAGAGTTCA (DR-1)......TTCTTCCTTCTAGActcgag