Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #8858)


Item Catalog # Description Quantity Price (USD)
Plasmid 8858 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5010
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    aP2 enhancer / promoter
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Fabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)
  • Tag / Fusion Protein
    • luciferase reporter (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid created by James Rhee by cloning 520bp of the aP2 enhancer from aP2-CAT into the pGL2 luciferase reporter. This is the abbreviated insert sequence:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    aP2-luciferase was a gift from Bruce Spiegelman (Addgene plasmid # 8858 ; ; RRID:Addgene_8858)
  • For your References section:

    Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Graves RA, Tontonoz P, Spiegelman BM. Mol Cell Biol. 1992 Mar . 12(3):1202-8. PubMed 1545801