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aP2-luciferase
(Plasmid #8858)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8858 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8858-D50 50 μg of DNA in Tris buffer $495
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DNA 8858-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL3-promoter
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5010
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aP2 enhancer / promoter
  • Alt name
    aP2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    520
  • GenBank ID
    M84651
  • Entrez Gene
    Fabp4 (a.k.a. 422/aP2, AFABP, ALBP, ALBP/Ap2, Ap2, Lbpl, P15)
  • Tag / Fusion Protein
    • luciferase reporter (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid created by James Rhee by cloning 520bp of the aP2 enhancer from aP2-CAT into the pGL2 luciferase reporter. This is the abbreviated insert sequence:
ggtaccGAATTCCAGCAGGAATCA.......GGATCAGAGTTCA (DR-1)......TTCTTCCTTCTAGActcgag

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    aP2-luciferase was a gift from Bruce Spiegelman (Addgene plasmid # 8858 ; http://n2t.net/addgene:8858 ; RRID:Addgene_8858)

  • For your References section:

    Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Graves RA, Tontonoz P, Spiegelman BM. Mol Cell Biol. 1992 Mar . 12(3):1202-8. PubMed 1545801
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