169,357 results

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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LDB-14-p65

Plasmid

#158985

Purpose

Expresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-14-p65

Tags

V5 tag , 6xHis tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-PB-R4R3

Plasmid

#139518

Purpose

Provides two PiggyBac transposon sequences between gene cassettes in the destination vector

Depositor

Minoru Ko

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2.aBTX

Plasmid

#122257

Purpose

Express membrane-tethered alpha-bungarotoxin in Danio rerio

Depositor

Loren Looger

Article

Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

Insert

alpha-bungarotoxin

Use

Synthetic Biology

Tags

FLAG

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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FUS-Citrine-PixE

Plasmid

#111505

Purpose

For PixELL system

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

PixE (slr1693 )

Use

Lentiviral

Tags

Citrine and FUS

Promoter

SFFV

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4

Plasmid

#107310

Purpose

Encoding dCas9-10xGCN4 driven by dSV40 promoter

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

dSV40-dCas9-10xGCN4

Use

Lentiviral

Expression

Mammalian

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLV V5-lamin B1

Plasmid

#175108

Purpose

Lentiviral expression of V5-tagged mouse Lmnb1

Depositor

Larry Gerace

Insert

Lmnb1 (Lmnb1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-Flag-UTR

Plasmid

#183235

Purpose

To express FLAG tagged eGFP

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

EGFP

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)/MBP-His

Plasmid

#38006

Depositor

Michael J Fox Foundation MJFF

Insert

MBP (Maltose binding protein)

Tags

6xHIS

Expression

Bacterial

Available Since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pMpGWB106

Plasmid

#68560

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4max-NRTH

Plasmid

#136919

Purpose

Expresses BE4max-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

Plasmid

#170048

Purpose

Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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GRM2-DuET

Plasmid

#213301

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GRM2 (GRM2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-N SV40 LT

Plasmid

#37857

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

SV40 LT (SV40gp6 Simian Virus 40)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PDL1

Plasmid

#223602

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_YFV_PrME

Plasmid

#207315

Purpose

Mammalian expression of Yellow fever virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

YFV_PrME (POLY Yellow fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.CAP-B22

Plasmid

#175005

Purpose

non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

Depositor

Viviana Gradinaru

Article

Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

Insert

Synthetic construct isolate AAV.CAP-B22 VP1 gene

Use

AAV

Expression

Mammalian

Mutation

7 amino acid substitution between VP1 452 and VP1…

Promoter

p41

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCIS GEM-CEPIA1er

Plasmid

#58217

Purpose

Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

GEM-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

GEM-GECO1 E298D F359W D400E E407D

Promoter

CAG

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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YFP-CAAX

Plasmid

#223558

Purpose

Plasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation box

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

YFP(Venus) - KRas4b

Expression

Mammalian

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mBFP-APPP1-mGFP

Plasmid

#196695

Purpose

As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

mEGFP and mtagBFP2

Expression

Mammalian

Mutation

Lys to Val substitution at position 612

Promoter

CMV

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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