163,479 results

1. pcDNA3.1-(empty)-TAG

   Plasmid

   #138209

   Purpose

   To facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' region

   Depositor

   Neal Devaraj

   Article

   Busby et al Methods Enzymol. 2020;641:373-399. doi:

   Type

   Empty backbone

   Use

   T7 transcription

   Tags

   TAG sequence

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter, T7 Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. B23(9-122)

   Plasmid

   #23142

   Depositor

   SeWon Suh

   Article

   Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

   Insert

   B23 core (NPM1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-8

   Promoter

   Available Since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pGP-CMV-GCaMP6f

   Plasmid

   #40755

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6f
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Halo-ATG9A HRD

   Plasmid

   #203222

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag flanked by human ATG9 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltra-U6-gRNAs1-5

   Plasmid

   #178569

   Purpose

   Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

   Depositor

   Christopher Lengner

   Article

   Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

   Insert

   5 gRNA-tRNA array with U6 promoter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER21-puro_TAOK3

   Plasmid

   #209778

   Purpose

   doxycycline-inducible expression of TAOK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK3 (TAOK3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-PKA-SPARK

   Plasmid

   #106920

   Purpose

   Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   PKA-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRibo-Sec-APEX2m

   Plasmid

   #176844

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Inducible

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIS2-TIP47 (Delta 1-190)

   Plasmid

   #17640

   Depositor

   James Hurley

   Article

   Hickenbottom et al Structure. 2004 Jul . 12(7):1199-207.

   Insert

   Tip47 (Delta 1-190) (Plin3 Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Delta 1-190

   Promoter

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pEntry_HCoV-OC43-nsp2

    Plasmid

    #168948

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-nsp2 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHRW-CRY2-VHH(GFP)

    Plasmid

    #126022

    Purpose

    express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CRY2 PHR-VHH(GFP)
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    WT

    Promoter

    Hsp70

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT-2

    Plasmid

    #22833

    Depositor

    Ryan Gill

    Article

    Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCP60 RS-GFP

    Plasmid

    #122173

    Purpose

    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

    Depositor

    Lukas Fischer

    Article

    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

    Insert

    RS-GFP
    

    Use

    Binary vector for escherichia coli and agrobacter…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNC-Cam2304-RNAi

    Plasmid

    #193400

    Purpose

    Nimble cloning-compatible vector for plant RNAi

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_WNT5A_WT

    Plasmid

    #82240

    Purpose

    Gateway Donor vector containing WNT5A , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    WNT5A (WNT5A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMTS-hFluc-HA-GFP11

    Plasmid

    #177726

    Purpose

    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    GFP11, HA, and Mitochondrial Targeting Sequence

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-MS2V5-eIF4G delN83

    Plasmid

    #65812

    Purpose

    expression of eIF4G-MS2 fusion.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eIF4G (EIF4G1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    delN83 (functionally behaves as the WT protein)

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. AbVec1.1-IGLC2-XhoI

    Plasmid

    #99575

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_YES1-1/1

    Plasmid

    #91439

    Purpose

    Protein expression and purification of human SH3 domain construct YES1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    YES1-1/1 (YES1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge