165,652 results

1. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

   Plasmid

   #158653

   Purpose

   making transgenics expressing a UAS driven mCherry and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Mutation

   F70A/F108Y

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG RpH-LAMP1-3xFLAG

   Plasmid

   #163018

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   RpH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHyo094 (eMutaT7)

   Plasmid

   #173124

   Purpose

   Expresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

   Insert

   pmCDA1-T7RNAP
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-6xGLY-His-tag-kanMX6

   Plasmid

   #20763

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Expression

   Yeast

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

   Plasmid

   #221357

   Purpose

   Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

   Plasmid

   #221358

   Purpose

   Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. AAV-CAG-GFP

   Plasmid

   #28014

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Insert

   eGFP
   

   Use

   AAV; Adeno associate viral

   Available Since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBI-p53 R175H/EGFP

   Plasmid

   #16544

   Depositor

   Bert Vogelstein

   Article

   Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

   Insert

   p53 (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R175H

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-lyso-pHoenix

   Plasmid

   #70112

   Purpose

   Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHoenix (Cd63 Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse)
   

   Tags

   Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS-RfxCas13d-HA

    Plasmid

    #141320

    Purpose

    Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    HA

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag TSC2

    Plasmid

    #14129

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 (TSC2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJC5-LAMP1-RFP-3xHA

    Plasmid

    #102932

    Purpose

    Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    RFP-HA

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-E-cadherin-RFP

    Plasmid

    #79603

    Purpose

    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    CDH1 promoter region (CDH1 Human)
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-miniTurbo

    Plasmid

    #124647

    Purpose

    Expresses Flag-miniTurbo fusion for proximity labeling

    Depositor

    Ken-Ichi Takemaru, FENG-QIAN Li

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBB541

    Plasmid

    #27394

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    groESL (groL, groS E. coli)
    

    Expression

    Bacterial

    Available Since

    March 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3HA-ERHBD-Hoxb8

    Plasmid

    #212932

    Purpose

    Expresses an estrogen-regulated form of Hoxb8 to generate conditionally immortalized hematopoietic progenitor cell lines

    Depositor

    Hans Haecker

    Article

    Redecke et al Nat Methods. 2013 Aug;10(8):795-803. doi

    Insert

    3HA-ERHBD-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Triple HA tag

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLAMP1-emiRFP670

    Plasmid

    #136570

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LAMP1-emiRFP670
    

    Tags

    emiRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cas9 TLS2

    Plasmid

    #196978

    Purpose

    Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS2 tag
    

    Expression

    Plant

    Promoter

    pG10-90

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-U6-sgRNA-hSyn-mCherry

    Plasmid

    #87916

    Purpose

    sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

    Depositor

    Alex Hewitt

    Article

    Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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