163,461 results

1. pJL1 sortase

   Plasmid

   #106288

   Purpose

   Expresses sortase in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   sortase A pentamutant
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH2930

   Plasmid

   #175283

   Purpose

   Pges-1 DAF-16a unc-54 3' UTR C.elegans gut expression of isoform a of DAF-16

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   DAF-16a (daf-16 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Pges-1

   Available Since

   Dec. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N3-FLuc-K0-Rluc_AH

   Plasmid

   #148846

   Purpose

   Mammalian Expression of K0-Rluc

   Depositor

   Elisa Izaurralde

   Insert

   K0-Rluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACOT4A

   Plasmid

   #42449

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ACOT4A (ACOT4 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. NbV5-eGFP

   Plasmid

   #201473

   Purpose

   Indirect microscopy/imaging of V5-tagged protein

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-eGFP
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-cerebody

   Plasmid

   #236738

   Purpose

   Protein overexpression of cerebody

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   cerebody
   

   Use

   Tags

   His, HA, FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hSyn_NES-Kinprola_PKA-mEGFP_WRPE-SV40

   Plasmid

   #233366

   Purpose

   hSyn1 driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   NES-Kinprola_PKA-mEGFP
   

   Use

   AAV

   Tags

   NES and mEGFP

   Expression

   Mutation

   Promoter

   hSyn1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

   Plasmid

   #216494

   Purpose

   Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAktKAR2.2
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

    Plasmid

    #216491

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Use

    Tags

    NES, T2A-EGFP-CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.3/TO/V5-DEST-mScarlet

    Plasmid

    #215536

    Purpose

    Lentiviral transfer vector to express the mScarlet fluorescent protein

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. 8061-E19

    Plasmid

    #234256

    Purpose

    Gateway ORF Entry clone of human BMPR1B with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    BMPR1B (BMPR1B Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 8061-E43

    Plasmid

    #234280

    Purpose

    Gateway ORF Entry clone of mouse Bmpr1b with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    Bmpr1b (Bmpr1b Mouse)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. P415 ymBaojin

    Plasmid

    #224086

    Purpose

    Expressing ymBaojin in yeast

    Depositor

    Bas Teusink

    Insert

    ymBaoJin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. KRD22_HBA1(HBA1reg-HBB-longHAs)

    Plasmid

    #232403

    Purpose

    AAV production plasmid for HBA1 long HAs vector from Figs. 3-6 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank full HBA1-2A-YFP cassette. HAs are ~900bp

    Depositor

    Matthew Porteus, Kyle Cromer

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Inserts

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Human Hemoglobin Subunit Beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MKC62_HBB(HBA1reg-HBA1-2A-YFP)

    Plasmid

    #232408

    Purpose

    AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.

    Depositor

    Kyle Cromer

    Article

    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

    Insert

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIVT-Bxb1

    Plasmid

    #232746

    Purpose

    IVT template for making modRNA encoding Bxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLBac-Bat-FluA-trimer

    Plasmid

    #229858

    Purpose

    Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

    Inserts

    PA_Bat-FluA_AFC35437.1
    PB1_Bat-FluA_AFC35436
    PB2_Bat-FluA_AFC35435.1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p27-mVenus reporter (PB)

    Plasmid

    #190731

    Purpose

    The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

    Depositor

    Toshiro Sato

    Article

    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

    Inserts

    mVenus
    p27
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    p27-F62A-F64A

    Promoter

    EF1α

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-GST-BcPC-PLC

    Plasmid

    #224255

    Purpose

    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

    Depositor

    Chiaki Murakami

    Article

    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

    Insert

    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    

    Use

    Tags

    GST and HRV-3C (LEVLFQGP) site

    Expression

    Bacterial

    Mutation

    The gene of Bacillus cereus ATCC 14579 was used f…

    Promoter

    lac promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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