169,357 results

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pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

Plasmid

#166804

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S1213A

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pXR002: EF1a-dCasRx-2A-EGFP

Plasmid

#109050

Purpose

Catalytically inactive dCasRx with 2A-EGFP

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

dCasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSV(X) C/EBPdelta

Plasmid

#13394

Depositor

Richard Schwartz

Article

Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

Insert

C/EBPdelta (Cebpd Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-EGFP

Plasmid

#176274

Purpose

Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHRW32

Plasmid

#130972

Purpose

HyPr plasmid with ble marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Expression

Yeast

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJA3

Plasmid

#19493

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA3 (DNAJA3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pZac2.1-gfaABC1D-PinkyCaMP

Plasmid

#232859

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

gfaABC1D

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-L4R1-tetO2

Plasmid

#139525

Purpose

Provides conditional promoter cassette between attL4-attR1 on Gateway entry vector

Depositor

Minoru Ko

Insert

tetO2 promoter

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-Dual-Guide-Donor-ShuttleOnly

Plasmid

#171098

Purpose

Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Mutation

XbaI site added upstream of U6 promotor. GtoC mu…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-153

Plasmid

#118567

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise

Plasmid

#103972

Purpose

Plasmid encoding mTurquoise

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-biRhoBAST

Plasmid

#196344

Purpose

Overexpression of circular biRhoBAST

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

Tornado-biRhoBAST

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pX335 HTT sgRNA-a

Plasmid

#87201

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

HTT (HTT Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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SFFV-CEBPB-Brd 226

Plasmid

#219169

Purpose

Transcription factor CEBPB with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

CEBPB (CEBPB Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-157

Plasmid

#118571

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX335 HTT sgRNA-b

Plasmid

#87200

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 5' UTR of HTT

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

HTT (HTT Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pNeae2

Plasmid

#168300

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Article

Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

Insert

Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus

Tags

6xHis, E-tag, and myc-tag

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINP

Plasmid

#41139

Depositor

Ray Owens

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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TFORF0107

Plasmid

#143010

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TRERF1 (TRERF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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