|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21577||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerD. Baltimore
- Backbone size w/o insert (bp) 9941
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsStable2 bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2500
Entrez GeneBmi1 (a.k.a. Bmi-1, Pcgf4)
/ Fusion Protein
- ires-eGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (destroyed during cloning)
- 3′ cloning site Not1 (partial digest) (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Bmi-1 cDNA cloned into pIRES-eGFP first. The Bmi1-ires-eGFP was then cut out and cloned into FUGW.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Bmi1-overexpression was a gift from Sally Temple (Addgene plasmid # 21577 ; http://n2t.net/addgene:21577 ; RRID:Addgene_21577)
For your References section:shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S. Cell Stem Cell. 2007 Jun 7. 1(1):87-99. 10.1016/j.stem.2007.04.001 PubMed 18371338