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Addgene

Bmi1-overexpression
(Plasmid #21577)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21577 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    D. Baltimore
  • Backbone size w/o insert (bp) 9941
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stable2 bacteria
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmi-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2500
  • GenBank ID
    NM_007552
  • Entrez Gene
    Bmi1 (a.k.a. Bmi-1, Pcgf4)
  • Tag / Fusion Protein
    • ires-eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site Not1 (partial digest) (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Bmi-1 cDNA cloned into pIRES-eGFP first. The Bmi1-ires-eGFP was then cut out and cloned into FUGW.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bmi1-overexpression was a gift from Sally Temple (Addgene plasmid # 21577 ; http://n2t.net/addgene:21577 ; RRID:Addgene_21577)
  • For your References section:

    shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S. Cell Stem Cell. 2007 Jun 7. 1(1):87-99. 10.1016/j.stem.2007.04.001 PubMed 18371338