|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4921
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNeuroligin 1
SpeciesM. musculus (mouse)
Insert Size (bp)2472
Entrez GeneNlgn1 (a.k.a. 6330415N05Rik, BB179718, N, NL1, Nl, Nlg1, mKIAA1070)
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
There is a A43V mutation in the aa acid sequence. The mutation is within the signal sequence and our data demonstrate that cleavage is normal. That means the mature protein does not have any change in amino acid sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-NL1(-) was a gift from Peter Scheiffele (Addgene plasmid # 15260 ; http://n2t.net/addgene:15260 ; RRID:Addgene_15260)
For your References section:Alternative splicing controls selective trans-synaptic interactions of the neuroligin-neurexin complex. Chih B, Gollan L, Scheiffele P. Neuron. 2006 Jul 20. 51(2):171-8. 10.1016/j.neuron.2006.06.005 PubMed 16846852