163,461 results

1. pLV-Syn1-mCherry

   Plasmid

   #222967

   Purpose

   Make lentivirus to express mCherry in syn1 expressing cells

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   synapsin I

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME map2k2a_s219D

   Plasmid

   #201058

   Purpose

   Gateway middle entry vector expressing constitutively active zebrafish map2k2a

   Depositor

   Sarah Childs

   Article

   Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

   Insert

   MAP2k2a (map2k2a Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Mutation S219D, constitutively active

   Promoter

   none

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR1051

   Plasmid

   #208739

   Purpose

   Characterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PZwf
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PZwf

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSR1057

   Plasmid

   #208742

   Purpose

   Characterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PPgk
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPgk

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF1067

   Plasmid

   #143920

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFATC2 (NFATC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2612

   Plasmid

   #144137

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF276 (ZNF276 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF1070

   Plasmid

   #142243

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFATC3 (NFATC3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N3-FLuc-K21-Rluc_AH

   Plasmid

   #148847

   Purpose

   Mammalian Expression of K21-Rluc

   Depositor

   Elisa Izaurralde

   Insert

   K21-Rluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_CRK_SH3

   Plasmid

   #178472

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CRK_SH3 (CRK Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH2198

    Plasmid

    #179773

    Purpose

    Prgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFP

    Depositor

    Mei Zhen

    Article

    Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

    Insert

    NLF-1::GFP (nlf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Prgef-1

    Available Since

    March 14, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Mouse 3' Ubf AID GFP PuroR

    Plasmid

    #127901

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Ubf Gene

    Depositor

    Mark Groudine

    Insert

    Ubf (Ubtf Mustard Weed, Mouse)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Ubf

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

    Plasmid

    #78507

    Purpose

    Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    Gads SH3 SH2 SH3 domain (GRAP2 Human)
    

    Use

    Tags

    GST followed by a prescission protease claevage s…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-C-mStayGold-Puro

    Plasmid

    #227253

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOSIP-KT (KanR, P21)

    Plasmid

    #45987

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRKH2

    Plasmid

    #71312

    Purpose

    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-CD86

    Plasmid

    #223604

    Purpose

    Lentiviral expression of human CD86 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FAH-rtTA3G

    Plasmid

    #120309

    Purpose

    An AAV vector that expresses rtTA3G under the c-fos promoter

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    rtTA3G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    c-fos

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLH01

    Plasmid

    #117261

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    PorfX-Lp_0640-42, sapK-sappR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HC20 pEYFPN1 anti-Homer1 nanobody

    Plasmid

    #135220

    Purpose

    Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC20 (Homer1 L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. opto-RhoA-mCherry_pcDNA3.1

    Plasmid

    #164472

    Purpose

    Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

    Insert

    opto-RhoA
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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