163,364 results

1. pRSV-PKImut-v2

   Plasmid

   #45067

   Purpose

   Expression of intactive PKI (PKA inhibitor) mutant

   Depositor

   Richard Maurer

   Insert

   cAMP-dependent protein kinase inhibitor alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Arg 20 & 21 to Glycines

   Promoter

   RSV

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet1-dCas9

   Plasmid

   #136650

   Purpose

   Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Tet1-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3` CC: Hygro – GFP-N – loxP – mScarlet

   Plasmid

   #219564

   Purpose

   3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   3` CC: Hygro – GFP-N – loxP – mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6P-1-GST-CD(Cbx7)

   Plasmid

   #82525

   Purpose

   Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 7 (CBX7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   CD(Cbx7) ; amino acids 1–62

   Promoter

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTHSSd_14

   Plasmid

   #59952

   Purpose

   Triple split (at 67, 601-SZ)

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   Three-fragment T7* RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTU-DO-HIS

   Plasmid

   #160005

   Purpose

   Low-copy expression vector for K. marxianus, histidine selection

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-C-Myc-DDK-IRES-Puro LARP7

   Plasmid

   #200725

   Purpose

   Lentiviral expression of Flag-Tagged human LARP7

   Depositor

   Claudio Alarcón

   Insert

   LARP7 (LARP7 Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-AGER-COMP5AP-AviTag-9xHis

   Plasmid

   #157325

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   AGER (AGER Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAID1.2-EF1a-CGFP-mAID

   Plasmid

   #140608

   Purpose

   Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMTU-DO-LEU

    Plasmid

    #160006

    Purpose

    Low-copy expression vector for K. marxianus, leucine selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PH-Akt-GFP

    Plasmid

    #51465

    Purpose

    Is a biosensor for PIP3/PI(3,4)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-pBAD

    Plasmid

    #54536

    Purpose

    Localization: Protein Expression Vector , Excitation: 568, Emission: 592

    Depositor

    Nathan Shaner, Roger Tsien, Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mApple

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBSV2_PresT-mCherryBb

    Plasmid

    #118238

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    resT promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOmniBac zz TEV YBBR TRF2

    Plasmid

    #185443

    Purpose

    Expresses human TRF2 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Insert

    TRF2 (TERF2 Human)
    

    Use

    Tags

    YBBR and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLys-M1 backbone

    Plasmid

    #109386

    Purpose

    Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBSV2_P0031-mCherryBb

    Plasmid

    #118240

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    bb0031 promoter driven mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-CHMP4A (1-204) (pGEX2T(TEV))

    Plasmid

    #80632

    Purpose

    Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-204

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Inducible Caspex expression

    Plasmid

    #97421

    Purpose

    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

    Depositor

    Samuel Myers, Steven Carr

    Article

    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

    Inserts

    Caspex
    T2A-Gfp
    

    Use

    CRISPR

    Tags

    3xFLAG and V5

    Expression

    Mammalian

    Mutation

    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

    Promoter

    TRE3G

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJFRC7-20XUAS-IVS-mCD8::GFP

    Plasmid

    #26220

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    mCD8::GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPN066

    Plasmid

    #91601

    Purpose

    Express sgRNA targeting human DPP4

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DPP4 (DPP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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