176,159 results

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pMIH-TFAM-GFP

Plasmid

#113704

Purpose

Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

Depositor

Benjamin Kile

Publication

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TFAM (Tfam Mouse)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Promoter

MSCV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Publication

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Expression

Mammalian

Promoter

8X Gli-binding site

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Publication

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Expression

Mammalian

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pUC18T-mini-Tn7T-Gm

Plasmid

#63121

Purpose

mini-Tn7 vector

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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C1(1-29)-miniTurbo-V5_pLX304

Plasmid

#107176

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Lentiviral

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV.Syn.GCaMP6f.WPRE.SV40

Plasmid

#100837

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Publication

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

131 Articles

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLPCX2-MT1-MMP-eGFP

Plasmid

#89819

Purpose

Express in MT1-MMP mammalian cells

Depositor

Shengyu Yang

Publication

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

MT1-MMP (MMP14 Human)

Use

Retroviral

Tags

eGFP

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-Nanoluc-ccdB

Plasmid

#87078

Purpose

Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Lentiviral

Tags

Nanoluc luciferase

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Publication

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Publication

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCI-MS2V5-PABPC1

Plasmid

#65807

Purpose

expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

Depositor

Niels Gehring

Publication

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PAB1 (PABPC1 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUC18T-mini-Tn7T-Tp-gfpmut3

Plasmid

#65033

Purpose

TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

gfpmut3

Expression

Bacterial

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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5'UTR CGG 99x FMR1-EGFP

Plasmid

#63091

Purpose

Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

Depositor

Nicolas Charlet-Berguerand

Insert

expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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mVenus-pBAD

Plasmid

#54845

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

6xHis and mVenus

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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EB1-tdTomato

Plasmid

#50825

Purpose

expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

Depositor

Erik Dent

Insert

EB1 (Mapre1 Mouse)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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